摩洛哥西北部人群和家禽中西尼罗河病毒感染的血清学证据。

Serological evidence of West Nile virus infection in human populations and domestic birds in the Northwest of Morocco.

机构信息

Institut Pasteur du Maroc, Service de Parasitologie et des Maladies Vectorielles, Place Louis Pasteur, Casablanca 20360, Morocco; Molecular Genetics and Immunophysiopathology Research Team, Health and Environment Laboratory, Aïn Chock Faculty of Sciences, University of Hassan II Casablanca (UH2C), Casablanca, Morocco.

Institut Pasteur du Maroc, Service de Parasitologie et des Maladies Vectorielles, Place Louis Pasteur, Casablanca 20360, Morocco.

出版信息

Comp Immunol Microbiol Infect Dis. 2021 Jun;76:101646. doi: 10.1016/j.cimid.2021.101646. Epub 2021 Apr 5.

DOI:10.1016/j.cimid.2021.101646

PMID:33845402

Abstract

West Nile virus (WNV) was recently detected in Culex pipiens mosquitoes in Morocco. The aim of this study was to evaluate the seroprevalence of WNV in humans and in domestic birds in two regions of Morocco by the detection of IgG antibodies. Blood samples were obtained from 91 human patients and 92 domestic birds from September to December 2019. All study samples were tested using competitive enzyme-linked immunosorbent assay (cELISA) and WNV neutralization tests (VNT) were performed on positive sera. Of all samples, 4 (4.39 %) humans and 4 (4.34 %) birds were found to be seropositive for flaviviruses by the cELISA test. The VNT revealed that three of the four human samples detected positive by cELISA contained neutralizing antibodies against WNV. Two bird samples were confirmed positive by VNT. These results show a significant seroprevalence of anti-WNV antibodies and therefore suggest the active circulation and exposure of human and bird populations in the northwest of Morocco.

摘要

西尼罗河病毒（WNV）最近在摩洛哥的库蚊中被检测到。本研究的目的是通过检测 IgG 抗体来评估摩洛哥两个地区人类和家禽中 WNV 的血清流行率。2019 年 9 月至 12 月期间，采集了 91 名人类患者和 92 只家禽的血液样本。所有研究样本均采用竞争酶联免疫吸附试验（cELISA）进行检测，对阳性血清进行了 WNV 中和试验（VNT）。通过 cELISA 试验，发现 4 名（4.39%）人类和 4 名（4.34%）鸟类的样本对黄病毒呈血清阳性。VNT 显示，在 cELISA 检测呈阳性的 4 个人类样本中，有 3 个样本含有针对 WNV 的中和抗体。两个鸟类样本通过 VNT 得到确认。这些结果表明存在针对 WNV 抗体的显著血清流行率，因此表明摩洛哥西北部的人类和鸟类群体存在活跃的循环和暴露。