RNA聚合酶II亚基3调节囊泡性癌过表达促生存蛋白1的表达以促进肝细胞癌。
RNA polymerase II subunit 3 regulates vesicular, overexpressed in cancer, prosurvival protein 1 expression to promote hepatocellular carcinoma.
作者信息
Hu Peng, Wang Binfeng, Chen Ting, Xu Yongfu, Zheng Guoqun, Zhu Yu, Du Xuefeng
机构信息
Department of Hepatobiliary Surgery, Taizhou Hospital of Zhejiang Province, Wenzhou Medical University, Linhai, Zhejiang, China.
Department of Hepatobiliary Surgery, Enze Hospital,Wenzhou Medical University, Taizhou, Zhejiang, China.
出版信息
J Int Med Res. 2021 Apr;49(4):300060521990512. doi: 10.1177/0300060521990512.
OBJECTIVE
To explore the relationships between hepatocellular carcinoma (HCC) and the expression of RNA polymerase II subunit 3 (RPB3) and vesicular, overexpressed in cancer, prosurvival protein 1 (VOPP1), and to determine whether RPB3 regulates VOPP1 expression to promote HCC cell proliferation, tumor growth, and tumorigenesis.
METHODS
HCC and adjacent liver samples were collected from 51 patients with HCC who underwent surgical excision between September 20, 2010 and June 22, 2017. Immunohistochemical staining, western blot, quantitative PCR, plate colony assay, and RNA microarray were used to detect relevant indexes for further analyses.
RESULTS
was shown to function as a target gene of RPB3 in facilitating HCC proliferation, and was downregulated after silencing. Additionally, hepatic tumor tissues demonstrated high VOPP1 expression. Furthermore, silencing suppressed tumor growth and cell proliferation and elicited apoptosis.
CONCLUSION
RPB3 regulates expression to promote HCC cell proliferation, tumor growth, and tumorigenesis.
目的
探讨肝细胞癌（HCC）与RNA聚合酶II亚基3（RPB3）和癌症中过表达的囊泡样促生存蛋白1（VOPP1）表达之间的关系，并确定RPB3是否通过调节VOPP1表达来促进肝癌细胞增殖、肿瘤生长和肿瘤发生。
方法
收集2010年9月20日至2017年6月22日期间接受手术切除的51例肝癌患者的肝癌及癌旁肝组织样本。采用免疫组织化学染色、蛋白质免疫印迹法、定量PCR、平板克隆实验和RNA微阵列检测相关指标以进行进一步分析。
结果
结果显示，在促进肝癌增殖过程中发挥RPB3靶基因的作用，沉默后其表达下调。此外，肝肿瘤组织中VOPP1表达较高。此外，沉默抑制肿瘤生长和细胞增殖并引发细胞凋亡。
结论
RPB3通过调节表达促进肝癌细胞增殖、肿瘤生长和肿瘤发生。
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