Department of Applied Chemistry, Faculty of Science and Engineering, Waseda University, 3-4-1 Ohkubo, Shinjuku-ku, Tokyo 169-8555, Japan.
Department of Applied Chemistry, Faculty of Science and Engineering, Waseda University, 3-4-1 Ohkubo, Shinjuku-ku, Tokyo 169-8555, Japan.
J Biosci Bioeng. 2021 Jul;132(1):18-24. doi: 10.1016/j.jbiosc.2021.03.001. Epub 2021 Apr 10.
2,5-Furandicarboxylic acid (FDCA) is a valuable compound that can be synthesized from biomass-derived hydroxymethylfurfural (HMF), and holds great potential as a promising replacement for petroleum-based terephthalic acid in the production of polyamides, polyesters, and polyurethanes used universally. However, an economical large-scale production strategy for HMF from lignocellulosic biomass is yet to be established. This study aimed to design a synthetic pathway that can yield FDCA from furfural, whose industrial production from lignocellulosic biomass has already been established. This artificial pathway consists of an oxidase and a prenylated flavin mononucleotide (prFMN)-dependent reversible decarboxylase, catalyzing furfural oxidation and carboxylation of 2-furoic acid, respectively. The prFMN-dependent reversible decarboxylase was identified in an isolated strain, Paraburkholderia fungorum KK1, whereas an HMF oxidase from Methylovorus sp. MP688 exhibited furfural oxidation activity and was used as a furfural oxidase. Using Escherichia coli cells coexpressing these proteins, as well as a flavin prenyltransferase, FDCA could be produced from furfural via 2-furoic acid in one pot.
2,5-呋喃二甲酸（FDCA）是一种有价值的化合物，可由生物质衍生的羟甲基糠醛（HMF）合成，作为石油基对苯二甲酸在聚酰胺、聚酯和普遍使用的聚氨酯生产中的有前途的替代品具有巨大的潜力。然而，从木质纤维素生物质中经济地大规模生产 HMF 的策略尚未建立。本研究旨在设计一种从糠醛生成 FDCA 的合成途径，糠醛已从木质纤维素生物质中工业化生产。该人工途径由氧化酶和依赖于 prenylated flavin mononucleotide (prFMN) 的可逆脱羧酶组成，分别催化糠醛氧化和 2-糠酸的羧化。依赖于 prFMN 的可逆脱羧酶在分离的菌株 Paraburkholderia fungorum KK1 中被鉴定出来，而来自 Methylovorus sp. MP688 的 HMF 氧化酶则表现出糠醛氧化活性，并被用作糠醛氧化酶。使用共表达这些蛋白质以及黄素 prenyltransferase 的大肠杆菌细胞，可以通过一锅法从糠醛经 2-糠酸生产 FDCA。
