Municipal Key Laboratory of Virology, Ningbo Municipal Center for Disease Control and Prevention, Ningbo 315010, PR China.
Anhui Province Key Laboratory of Veterinary Pathobiology and Disease Control, College of Animal Science and Technology, Anhui Agricultural University, Hefei 230036, PR China.
Pol J Vet Sci. 2021 Mar;24(1):43-49. doi: 10.24425/pjvs.2021.136791.
In this study, we developed a SYBR Green I real-time PCR method for the rapid and sensitive detection of novel porcine parvovirus 7 (PPV7). Specific primers were designed based on the highly conserved region within the Capsid gene of PPV7. The established method was 1,000 times more sensitive than the conventional PCR method and had a detection limit of 35.6 copies. This method was specific and had no cross-reactions with PCV2, PCV3, PRV, PEDV, PPV1, and PPV6. Experiments testing the intra and interassay precision demonstrated a high reproducibility. Testing the newly established method with 200 clinical samples revealed a detection rate up to 17.5% higher than that of the conventional PCR assay. The established method could provide technical support for clinical diagnosis and epidemiological investigation of PPV7.
在这项研究中，我们开发了一种 SYBR Green I 实时 PCR 方法，用于快速灵敏地检测新型猪细小病毒 7（PPV7）。根据 PPV7 衣壳基因内的高度保守区域设计了特异性引物。建立的方法比常规 PCR 方法灵敏 1000 倍，检测限为 35.6 拷贝。该方法具有特异性，与 PCV2、PCV3、PRV、PEDV、PPV1 和 PPV6 无交叉反应。测试内和间试验精度的实验表明具有很高的重现性。用 200 个临床样本测试新建立的方法，其检测率比常规 PCR 方法高 17.5%。该建立的方法可为 PPV7 的临床诊断和流行病学调查提供技术支持。
