脱氧费加诺霉素D对耻垢分枝杆菌ATCC 607的作用模式。

Mode of action of deoxypheganomycin D on Mycobacterium smegmatis ATCC 607.

作者信息

Suzukake-Tsuchiya K, Hori M, Shimada N, Hamada M

机构信息

Showa College of Pharmaceutical Sciences, Tokyo, Japan.

出版信息

J Antibiot (Tokyo). 1988 May;41(5):675-83. doi: 10.7164/antibiotics.41.675.

DOI:10.7164/antibiotics.41.675

PMID:3384753

Abstract

Deoxypheganomycin D, a specific inhibitor of mycobacteria, inhibits the growth in vitro of Mycobacterium smegmatis ATCC 607 (M. 607) bacteriostatically at concentrations as high as 7 X 10(-5) M. It shows no cross-resistance to paromomycin, capreomycin, viomycin, streptothricin, kanamycin and streptomycin. Deoxypheganomycin D at 2.8 X 10(-7) M where the cell growth of M. 607 is only partially inhibited does not significantly inhibit DNA, RNA or protein synthesis but leads to marked decrease (13% of control) in [14C]glycerol-derived radioactivity in cell-walls. In the presence of 7 X 10(-6) M deoxypheganomycin D, the influx of leucine but not thymidine is affected while the reverse is true with efflux. The data suggest that the effect of deoxypheganomycin D on M. 607 may be related to both the cell membrane and specific mycobacterial lipid like components of the cell-wall.

摘要

脱氧费加诺霉素D是一种分枝杆菌特异性抑制剂，在浓度高达7×10⁻⁵ M时对耻垢分枝杆菌ATCC 607（M. 607）具有抑菌作用。它对巴龙霉素、卷曲霉素、紫霉素、链丝菌素、卡那霉素和链霉素无交叉耐药性。在2.8×10⁻⁷ M的脱氧费加诺霉素D作用下，M. 607的细胞生长仅受到部分抑制，此时它对DNA、RNA或蛋白质合成无显著抑制作用，但导致细胞壁中[¹⁴C]甘油衍生放射性显著降低（为对照的13%）。在7×10⁻⁶ M脱氧费加诺霉素D存在的情况下，亮氨酸的流入受到影响，但胸苷不受影响，而流出情况则相反。数据表明，脱氧费加诺霉素D对M. 607的作用可能与细胞膜以及细胞壁中特定的分枝杆菌脂质样成分有关。