Department of Microbiology, Faculty of Biological Sciences, Alzahra University, Tehran, Iran.
Microbial Biotechnology and Bioprocess Engineering (MBBE) Group, Department of Microbiology, Faculty of Science, University of Maragheh, Maragheh, Iran.
Methods Mol Biol. 2021;2307:221-232. doi: 10.1007/978-1-0716-1414-3_15.
Yarrowia lipolytica produces a range of valuable biotechnological products from natural metabolites and enzymes to heterologous proteins. The production of these products is affected by medium composition and various environmental factors. Here we describe bioprocess development for a recombinant laccase production by Y. lipolytica. At first, response surface methodology (RSM), as a statistical technique for design of experiment (DOE), is used for the optimization of medium composition in flask level. Then, results of RSM are applied to increase laccase production in controlled conditions of the bioreactor.
解脂耶氏酵母能够从天然代谢产物和酶到异源蛋白生产出一系列有价值的生物技术产品。这些产品的生产受到培养基组成和各种环境因素的影响。在这里，我们描述了通过解脂耶氏酵母生产重组漆酶的生物工艺开发。首先，响应面法（RSM）作为实验设计（DOE）的统计技术，用于优化摇瓶水平的培养基组成。然后，将 RSM 的结果应用于在生物反应器的控制条件下提高漆酶的产量。
