绿弯菌中2-氧代-3-甲基戊酸的生物合成机制。
Mechanism of biosynthesis of 2-oxo-3-methylvalerate in Chlorobium vibrioforme.
作者信息
Nesbakken T, Kolsaker P, Ormerod J
机构信息
Department of Biology, University of Oslo, Norway.
出版信息
J Bacteriol. 1988 Jul;170(7):3287-90. doi: 10.1128/jb.170.7.3287-3290.1988.
Abstract
The biosynthesis of 2-oxo-3-methylvalerate in Chlorobium vibrioforme was investigated by 13C nuclear magnetic resonance spectroscopy of the oxoacid formed from 13C-labeled acetate by washed suspensions. The threonine pathway could be excluded, and the results are in accord with a mechanism for the formation of 2-oxobutyrate from acetyl coenzyme A and pyruvate via citramalate.
摘要
通过对由经洗涤的悬浮液中13C标记的乙酸盐形成的含氧酸进行13C核磁共振光谱分析，研究了绿弯菌中2-氧代-3-甲基戊酸的生物合成。可以排除苏氨酸途径，结果与通过柠苹酸从乙酰辅酶A和丙酮酸形成2-氧代丁酸的机制一致。
相似文献
1
Mechanism of biosynthesis of 2-oxo-3-methylvalerate in Chlorobium vibrioforme.绿弯菌中2-氧代-3-甲基戊酸的生物合成机制。
J Bacteriol. 1988 Jul;170(7):3287-90. doi: 10.1128/jb.170.7.3287-3290.1988.
2
Carbon dioxide--fixation in photosynthetic green sulfur bacteria.光合绿色硫细菌中的二氧化碳固定
Science. 1970 Jul 10;169(3941):186-8. doi: 10.1126/science.169.3941.186.
3
13C-NMR study of acetate assimilation in Thermoproteus neutrophilus.嗜中性嗜热栖热菌中乙酸同化作用的13C-核磁共振研究。
Eur J Biochem. 1989 Dec 22;186(3):695-700. doi: 10.1111/j.1432-1033.1989.tb15262.x.
4
Amino acid biosynthesis in the halophilic archaeon Haloarcula hispanica.嗜盐古菌西班牙盐盒菌中的氨基酸生物合成。
J Bacteriol. 1999 May;181(10):3226-37. doi: 10.1128/JB.181.10.3226-3237.1999.
5
Carbon dioxide fixation in green sulphur bacteria.绿硫细菌中的二氧化碳固定
Biochem J. 1970 Nov;120(2):399-408. doi: 10.1042/bj1200399.
6
Biosynthesis of the diterpene verrucosan-2beta-ol in the phototrophic eubacterium Chloroflexus aurantiacus. A retrobiosynthetic NMR study.光合真细菌橙色绿屈挠菌中二萜疣孢菌素 - 2β - 醇的生物合成。一项逆生物合成核磁共振研究。
J Biol Chem. 1998 Jul 17;273(29):18099-108. doi: 10.1074/jbc.273.29.18099.
7
[Formation of keto acids by barotolerant marine bacteria under atmospheric pressure].耐压海洋细菌在常压下形成酮酸
Mikrobiologiia. 1975 Mar-Apr;44(2):369-71.
8
Assessment of the flux of mitochondrial acetyl-CoA in liver and kidney by using the differential production of 14CO2 from tracers of (1-14C)- and (2-14C)-labelled 4-methyl-2-oxovalerate.通过使用(1-¹⁴C)-和(2-¹⁴C)-标记的4-甲基-2-氧代戊酸示踪剂产生的¹⁴CO₂差异来评估肝脏和肾脏中线粒体乙酰辅酶A的通量。
Biochem J. 1983 Jan 15;210(1):265-8. doi: 10.1042/bj2100265.
9
Mevalonic acid is partially synthesized from amino acids in Halobacterium cutirubrum: a 13C nuclear magnetic resonance study.甲羟戊酸在深红嗜盐菌中部分由氨基酸合成：一项碳-13核磁共振研究。
J Bacteriol. 1986 May;166(2):559-64. doi: 10.1128/jb.166.2.559-564.1986.
10
Eliminating acetate formation improves citramalate production by metabolically engineered Escherichia coli.通过代谢工程改造的大肠杆菌消除乙酸盐形成可提高苹果酸柠檬酸酯的产量。
Microb Cell Fact. 2017 Jun 21;16(1):114. doi: 10.1186/s12934-017-0729-2.
引用本文的文献
1
Citramalate synthase yields a biosynthetic pathway for isoleucine and straight- and branched-chain ester formation in ripening apple fruit.柠檬酸盐合酶为成熟苹果果实中异亮氨酸和直链及支链酯的生物合成途径提供了一条途径。
Proc Natl Acad Sci U S A. 2021 Jan 19;118(3). doi: 10.1073/pnas.2009988118.
2
An enzyme and(13)C-NMR study of carbon metabolism in heliobacteria.一种产甲烷菌碳代谢的酶和（13）C-NMR 研究。
Photosynth Res. 1994 Jul;41(1):75-88. doi: 10.1007/BF02184147.
本文引用的文献
1
Origin of the two peaks for 2-keto-3-methylvaleric acid produced by the oximation of the keto acids occurring in maple syrup urine disase.枫糖尿症中酮酸肟化产生的2-酮-3-甲基戊酸两个峰的起源。
J Chromatogr. 1980 May 9;182(2):221-5. doi: 10.1016/s0378-4347(00)81626-6.
2
Biosynthetic pathways in Methanospirillum hungatei as determined by 13C nuclear magnetic resonance.通过碳-13核磁共振确定的亨氏甲烷螺菌的生物合成途径。
J Bacteriol. 1983 Oct;156(1):316-26. doi: 10.1128/jb.156.1.316-326.1983.
3
Multiple pathways for isoleucine biosynthesis in the spirochete Leptospira.钩端螺旋体中异亮氨酸生物合成的多种途径。
J Bacteriol. 1983 May;154(2):846-53. doi: 10.1128/jb.154.2.846-853.1983.
4
Photoassimilation of acetate and the biosynthesis of amino acids by Chlorobium thiosulphatophilum.嗜硫代硫酸盐绿菌对乙酸盐的光同化作用及氨基酸的生物合成
Biochem J. 1964 Jun;91(3):546-59. doi: 10.1042/bj0910546.
5
6
Metabolism of beta-methylaspartate by a pseudomonad.假单胞菌对β-甲基天冬氨酸的代谢
J Bacteriol. 1968 Dec;96(6):2094-8. doi: 10.1128/jb.96.6.2094-2098.1968.
7
Carbon dioxide fixation in green sulphur bacteria.绿硫细菌中的二氧化碳固定
Biochem J. 1970 Nov;120(2):399-408. doi: 10.1042/bj1200399.
8
A new ferredoxin-dependent carbon reduction cycle in a photosynthetic bacterium.光合细菌中一种新的依赖铁氧化还原蛋白的碳还原循环。
Proc Natl Acad Sci U S A. 1966 Apr;55(4):928-34. doi: 10.1073/pnas.55.4.928.
9
Further studies on carbon dioxide fixation in Chlorobium.绿硫菌中二氧化碳固定的进一步研究。
Arch Mikrobiol. 1974 Jun 7;98(1):3-18. doi: 10.1007/BF00425263.
10
Quantitative analysis of branched-chain alpha-keto acids as their trimethylsilylated oximes.
Clin Chim Acta. 1973 Oct 30;48(3):279-85. doi: 10.1016/0009-8981(73)90197-6.
Zotero 插件