Nesbakken T, Kolsaker P, Ormerod J
Department of Biology, University of Oslo, Norway.
J Bacteriol. 1988 Jul;170(7):3287-90. doi: 10.1128/jb.170.7.3287-3290.1988.
The biosynthesis of 2-oxo-3-methylvalerate in Chlorobium vibrioforme was investigated by 13C nuclear magnetic resonance spectroscopy of the oxoacid formed from 13C-labeled acetate by washed suspensions. The threonine pathway could be excluded, and the results are in accord with a mechanism for the formation of 2-oxobutyrate from acetyl coenzyme A and pyruvate via citramalate.
通过对由经洗涤的悬浮液中13C标记的乙酸盐形成的含氧酸进行13C核磁共振光谱分析，研究了绿弯菌中2-氧代-3-甲基戊酸的生物合成。可以排除苏氨酸途径，结果与通过柠苹酸从乙酰辅酶A和丙酮酸形成2-氧代丁酸的机制一致。
