通过使用(1-¹⁴C)-和(2-¹⁴C)-标记的4-甲基-2-氧代戊酸示踪剂产生的¹⁴CO₂差异来评估肝脏和肾脏中线粒体乙酰辅酶A的通量。
Assessment of the flux of mitochondrial acetyl-CoA in liver and kidney by using the differential production of 14CO2 from tracers of (1-14C)- and (2-14C)-labelled 4-methyl-2-oxovalerate.
作者信息
Tomera J F, Kopito R R, Brunengraber H
出版信息
Biochem J. 1983 Jan 15;210(1):265-8. doi: 10.1042/bj2100265.
Abstract
A procedure is described to convert rates of (14)CO(2) production into rates of mitochondrial acetyl-CoA production from a (14)C-labelled substrate. The principle is illustrated in perfused rat liver and kidney by the differential yield of (14)CO(2) from 4-methyl-2-oxo[1-(14)C]valerate and 4-methyl-2-oxo[2-(14)C]valerate.
摘要
本文描述了一种将（14）CO₂生成速率转化为来自（14）C标记底物的线粒体乙酰辅酶A生成速率的方法。通过4-甲基-2-氧代[1-（14）C]戊酸和4-甲基-2-氧代[2-（14）C]戊酸产生（14）CO₂的差异产量，在灌注大鼠肝脏和肾脏中阐明了该原理。
相似文献
1
Assessment of the flux of mitochondrial acetyl-CoA in liver and kidney by using the differential production of 14CO2 from tracers of (1-14C)- and (2-14C)-labelled 4-methyl-2-oxovalerate.通过使用(1-¹⁴C)-和(2-¹⁴C)-标记的4-甲基-2-氧代戊酸示踪剂产生的¹⁴CO₂差异来评估肝脏和肾脏中线粒体乙酰辅酶A的通量。
Biochem J. 1983 Jan 15;210(1):265-8. doi: 10.1042/bj2100265.
2
Rates of catabolism calculated from 14CO2 production: artifacts and realities.根据二氧化碳生成量计算的分解代谢率：假象与事实
Pharmacol Biochem Behav. 1983;18 Suppl 1:285-8. doi: 10.1016/0091-3057(83)90187-9.
3
Interaction of short-chain and branched-chain fatty acids and their carnitine and CoA esters and of various metabolites and agents with branched-chain 2-oxo acid oxidation in rat muscle and liver mitochondria.短链脂肪酸和支链脂肪酸及其肉碱酯和辅酶A酯与各种代谢物和试剂对大鼠肌肉和肝脏线粒体中支链2-氧代酸氧化的相互作用。
Int J Biochem. 1985;17(9):967-74. doi: 10.1016/0020-711x(85)90241-1.
4
Oxidation of 2-oxoisocaproate and 2-oxoisovalerate by the perfused rat heart. Interactions with fatty acid oxidation.灌注大鼠心脏对2-氧代异己酸和2-氧代异戊酸的氧化。与脂肪酸氧化的相互作用。
Biochem Cell Biol. 1990 Jan;68(1):260-5. doi: 10.1139/o90-036.
5
A study of the metabolism of [U-14C]3-methyl-2-oxopentanoate by rat liver mitochondria using h.p.l.c. with continuous on-line monitoring of radioactive intact acyl-coenzyme A intermediates.一项利用高效液相色谱法并对放射性完整酰基辅酶 A 中间体进行连续在线监测，研究大鼠肝脏线粒体对[U-14C]3-甲基-2-氧代戊酸酯代谢的实验。
Biochem J. 1986 Apr 15;235(2):343-50. doi: 10.1042/bj2350343.
6
The shunt pathway of mevalonate metabolism in the isolated perfused rat liver.离体灌注大鼠肝脏中甲羟戊酸代谢的分流途径。
J Biol Chem. 1984 Jul 25;259(14):8939-44.
7
Analysis of tricarboxylic acid-cycle metabolism of hepatoma cells by comparison of 14CO2 ratios.通过比较14CO2比率分析肝癌细胞的三羧酸循环代谢
Biochem J. 1987 Sep 15;246(3):633-9. doi: 10.1042/bj2460633.
8
The shunt pathway of mevalonate metabolism in the isolated perfused rat kidney.离体灌注大鼠肾脏中甲羟戊酸代谢的分流途径。
J Biol Chem. 1984 Jan 10;259(1):372-7.
9
10
Pathways of acetoacetate's formation in liver and kidney.肝脏和肾脏中乙酰乙酸的形成途径。
J Biol Chem. 1982 Aug 25;257(16):9290-3.
引用本文的文献
1
The stimulation of tricarboxylic acid-cycle flux by alpha-adrenergic agonists in perfused rat liver.α-肾上腺素能激动剂对灌注大鼠肝脏三羧酸循环通量的刺激作用。
Biochem J. 1986 Jan 15;233(2):321-4. doi: 10.1042/bj2330321.
本文引用的文献
1
Conversion of fatty acids to carbohydrate; application of isotopes to this problem and role of the Krebs cycle as a synthetic pathway.脂肪酸向碳水化合物的转化；同位素在该问题中的应用以及三羧酸循环作为合成途径的作用。
Physiol Rev. 1957 Apr;37(2):252-72. doi: 10.1152/physrev.1957.37.2.252.
2
Synthesis via the Krebs' cycle in the utilization of acetate by rat liver slices.大鼠肝切片利用乙酸盐时通过三羧酸循环进行的合成。
Biochim Biophys Acta. 1955 Sep;18(1):87-101. doi: 10.1016/0006-3002(55)90012-3.
3
Studies on the regulation of the branched chain alpha-keto acid dehydrogenase in the perfused rat liver.灌注大鼠肝脏中支链α-酮酸脱氢酶的调节研究。
J Biol Chem. 1981 Sep 10;256(17):9009-15.
4
Subcellular distribution and partial characterization of an alpha-ketoisocaproate oxidase of rat liver: formation of beta-hydroxyisovaleric acid.大鼠肝脏α-酮异己酸氧化酶的亚细胞分布及部分特性：β-羟基异戊酸的形成
Arch Biochem Biophys. 1981 Jan;206(1):132-44. doi: 10.1016/0003-9861(81)90074-6.
5
Binding of branched-chain 2-oxo acids to bovine serum albumin.支链2-氧代酸与牛血清白蛋白的结合。
Biochem J. 1982 Apr 15;204(1):265-72. doi: 10.1042/bj2040265.
6
Lipogenesis from ketone bodies in the isolated perfused rat liver. Evidence for the cytosolic activation of acetoacetate.离体灌注大鼠肝脏中酮体的脂肪生成。乙酰乙酸胞质活化的证据。
J Biol Chem. 1982 Apr 10;257(7):3434-40.
7
Regulation of alpha-ketoisocaproate binding to albumin in vivo by free fatty acids.游离脂肪酸对体内α-酮异己酸与白蛋白结合的调节作用。
Am J Physiol. 1982 Jan;242(1):E67-71. doi: 10.1152/ajpendo.1982.242.1.E67.
8
Regulation of the branched chain alpha-ketoacid pathway in liver.肝脏中支链α-酮酸途径的调节
J Biol Chem. 1982 Aug 25;257(16):9668-76.
9
Underestimation of metabolic rates owing to reincorporation of 14CO2 in the perfused rat liver.由于灌注大鼠肝脏中14CO2的再掺入导致代谢率低估。
Biochem J. 1982 Oct 15;208(1):231-4. doi: 10.1042/bj2080231.
10
Metabolic activities of the isolated perfused rat kidney.离体灌注大鼠肾脏的代谢活动。
Biochem J. 1967 Jun;103(3):852-62. doi: 10.1042/bj1030852.
Zotero 插件