雌激素连接亚硝基脲在甲基亚硝基脲诱导的大鼠乳腺癌中的体内和体外活性比较。
Comparison of in vivo and in vitro activity of estrogen-linked nitrosoureas in methylnitrosourea-induced rat mammary carcinoma.
作者信息
Petru E, Berger M R, Kaufmann M
机构信息
Institute of Toxicology and Chemotherapy, German Cancer Research Center, Heidelberg.
出版信息
J Cancer Res Clin Oncol. 1988;114(3):317-20. doi: 10.1007/BF00405842.
The anticancer activity of estradiol-linked 2-chloroethylnitrosoureas in methylnitrosourea-induced rat mammary carcinoma was investigated using the short-term assay and the bilayer soft agar system after in vitro exposure as well as the monolayer methylcellulose assay following in vivo treatment. The aim was to evaluate to what extent previous in vivo findings are paralleled by the results of two in vitro test systems and the monolayer culture technique ex vivo. From the test systems investigated and under the conditions used, the results of the short-term test and the monolayer methylcellulose assay did not show a correlation with previous in vivo findings. Only the results of the bilayer clonogenic assay paralleled therapeutic parameters in vivo, although the degree of activity obtained in vivo was not reflected in vitro.
使用短期试验和体外暴露后的双层软琼脂系统以及体内治疗后的单层甲基纤维素试验，研究了雌二醇连接的2-氯乙基亚硝基脲在甲基亚硝基脲诱导的大鼠乳腺癌中的抗癌活性。目的是评估两个体外测试系统和离体单层培养技术的结果在多大程度上与先前的体内研究结果相似。在所研究的测试系统和所用条件下，短期试验和单层甲基纤维素试验的结果与先前的体内研究结果没有相关性。只有双层克隆形成试验的结果与体内治疗参数相似，尽管体内获得的活性程度在体外没有体现出来。
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