Song Jianbo, Mao Hanyi, Cheng Jie, Zhou Yong, Chen Rongrong, Zeng Liming, Li Hua, Wang Yihua
College of Biological Sciences and Engineering, Jiangxi Agricultural University, Nanchang 330045, PR China.
College of Life Science, Henan Agricultural University, Zhengzhou 450002, PR China.
Gene. 2021 Jun 30;787:145641. doi: 10.1016/j.gene.2021.145641. Epub 2021 Apr 18.
Trehalose-6-phosphate synthase (TPS) exerts important functions related to plant desiccation tolerance and responses to environmental stimuli. However, in Medicago truncatula, the TPS family has not been reported to date. This study found 11 MtTPS genes in the genome of M. truncatula, which could be divided into two subfamilies: Class I and Class II. All TPS family members have a TPS domain (Glyco transf_20) at the N-terminus and a TPP domain (Trehalose_PPase) at the C-terminus. Interestingly, the genetic structures differ between Class I and Class II, Class I members have more introns than Class II members. Furthermore, transcriptome and real-time PCR analysis showed that five MtTPS genes could be induced by drought, salt or cold. Specifically, MtTPS2, MtTPS8, MtTPS9, MtTPS11 were up-regulated under both drought and salt treatment, particularly, MtTPS8 and MtTPS9 can also be induced by cold, while MtTPS7 only responded to salt stress. In summary, this study provides the foundation for further research on TPS genes in M. truncatula and their regulatory function in response to abiotic stresses.
海藻糖-6-磷酸合酶（TPS）发挥着与植物耐旱性以及对环境刺激的响应相关的重要功能。然而，截至目前，在蒺藜苜蓿中尚未有关于TPS家族的报道。本研究在蒺藜苜蓿基因组中发现了11个MtTPS基因，它们可分为两个亚家族：I类和II类。所有TPS家族成员在N端都有一个TPS结构域（糖基转移酶_20），在C端都有一个TPP结构域（海藻糖磷酸酶）。有趣的是，I类和II类的基因结构有所不同，I类成员比II类成员具有更多的内含子。此外，转录组和实时PCR分析表明，5个MtTPS基因可被干旱、盐或冷诱导。具体而言，MtTPS2、MtTPS8、MtTPS9、MtTPS11在干旱和盐处理下均上调，特别是MtTPS8和MtTPS9也可被冷诱导，而MtTPS7仅对盐胁迫有响应。总之，本研究为进一步研究蒺藜苜蓿中的TPS基因及其在应对非生物胁迫中的调控功能奠定了基础。
