Wang Xiaolong, Chai Hua, Li Shasha, Xu Yanxia, Wu Yue, Wang Jianli, Yang Zhao
Branch of Animal Husbandry and Veterinary of Heilongjiang Academy of Agricultural Sciences, Qiqihar, Heilongjiang, China.
Institute of Grass Science of Heilongjiang Academy of Agricultural Sciences, Harbin, Heilongjiang, China.
Front Plant Sci. 2024 Dec 9;15:1486564. doi: 10.3389/fpls.2024.1486564. eCollection 2024.
Alfalfa, scientifically identified as , is repeatedly referred to as the "king of forages". Because of its tight relationship to winter hardiness, the alfalfa's root crown plays a significant role as a storage organ over the winter. At present, it is still unknown what molecular process makes the alfalfa root crown resistant to cold. This study was aimed to study these knowledge gaps. Using RNA sequencing (RNA-Seq) technology, significant genes associated with cold hardiness were found.
According to the random block design, Longmu 806 alfalfa and Sardi alfalfa were planted in regional experiments. Under the condition of low-temperature treatment in winter, the differentially expressed genes (DEGs), winter survival rate (WSR), and physiological characteristics were, in turn, calculated by RNA-Seq, chemical analysis, and field investigation.
The WSR of the Longmu 806 alfalfa was 3.68-fold greater than that of the Sardi alfalfa. The jasmonic acid (JA), soluble sugar (SS), proline (Pro), and glutathione (GSH) concentration in the roots of Longmu 806 alfalfa was more than the same amount in Sardi alfalfa in other words P is less than 0.05. An entire set of 878 DEGs related to winter hardiness was found by statistical analysis. Among them, 463 DEGs showed an increase in expression, whereas 415 DEGs showed a decrease in expression. The metabolic pathways' examination presented that the DEGs (, , and ) were linked to the pathways of "plant hormone signaling transduction", "Amino sugar and nucleotide sugar metabolism", and "glutathione metabolism". Furthermore, the physiological changes in JA, SS, Pro content, and GSH were influenced by the dynamic transcription profile of LT (low- temperature) resistance-related genes.
紫花苜蓿，科学上鉴定为 ，常被称为“牧草之王”。由于其与抗寒性密切相关，紫花苜蓿的根颈作为越冬贮藏器官起着重要作用。目前，尚不清楚是什么分子过程使紫花苜蓿根颈具有抗寒能力。本研究旨在填补这些知识空白。利用RNA测序（RNA-Seq）技术，发现了与抗寒性相关的重要基因。
按照随机区组设计，将龙牧806苜蓿和萨迪苜蓿进行区域试验种植。在冬季低温处理条件下，依次通过RNA-Seq、化学分析和田间调查计算差异表达基因（DEGs）、越冬存活率（WSR）和生理特性。
龙牧806苜蓿的WSR比萨迪苜蓿高3.68倍。龙牧806苜蓿根中茉莉酸（JA）、可溶性糖（SS）、脯氨酸（Pro）和谷胱甘肽（GSH）的浓度高于萨迪苜蓿，即P<0.05。通过统计分析发现了一整套878个与抗寒性相关的DEGs。其中，463个DEGs表达增加，而415个DEGs表达减少。代谢途径分析表明，DEGs（ 、 和 ）与“植物激素信号转导”、“氨基糖和核苷酸糖代谢”以及“谷胱甘肽代谢”途径相关。此外，JA、SS、Pro含量和GSH的生理变化受低温（LT）抗性相关基因动态转录谱的影响。
