Soltani Zangbar Hamid, Shahabi Parviz, Seyedi Vafaee Manouchehr, Ghadiri Tahereh, Ebrahimi Kalan Abbas, Fallahi Solmaz, Ghorbani Meysam, Jafarzadehgharehziaaddin Mohsen
Department of Neuroscience and Cognition, Faculty of Advanced Medical Sciences, Tabriz University of Medical Sciences, Tabriz, Iran; Aging Research Institute, Tabriz University of Medical Sciences, Tabriz, Iran; Neurosciences Research Centre (NSRC), Tabriz University of Medical Sciences, Tabriz, Iran.
Aging Research Institute, Tabriz University of Medical Sciences, Tabriz, Iran; Neurosciences Research Centre (NSRC), Tabriz University of Medical Sciences, Tabriz, Iran; Department of Physiology, Faculty of Medicine, Tabriz University of Medical Sciences, Tabriz, Iran.
Brain Res Bull. 2021 Jul;172:31-42. doi: 10.1016/j.brainresbull.2021.04.004. Epub 2021 Apr 10.
Spinal Cord Injury (SCI), triggers neurodegenerative changes in the spinal cord, and simultaneously alters oscillatory manifestations of motor cortex. However, these disturbances may not be limited to motor areas and other parts such as hippocampus, which is vital in the neurogenesis and cognitive function, may be affected in the neurogenic and oscillatory manners. Addressing this remarkable complication of SCI, we evaluated the hippocampal neurogenesis and rhythms through acute phase of SCI. In the present study, we used 40 male rats (Sham.W1 = 10, SCI.W1 = 10, Sham.W2 = 10, SCI.W2 = 10), and findings revealed that contusive SCI declines hippocampal rhythms (Delta, Theta, Beta, Gamma) power and max-frequency. Also, there was a significant decrease in the DCX + and BrdU + cells of the dentate gyrus; correlated significantly with rhythms power decline. Considering the TUNEL assay analysis, there were significantly greater apoptotic cells, in the CA1, CA3, and DG regions of injured animals. Furthermore, according to the western blotting analysis, the expression of receptors (NMDA, GABAA, Muscarinic1), which are essential in the neurogenesis and generation of rhythms significantly attenuated following SCI. Our study demonstrated that acute SCI, alters the power and max-frequency of hippocampal rhythms parallel with changes in the hippocampal neurogenesis, apoptosis, and receptors expression.
脊髓损伤（SCI）会引发脊髓中的神经退行性变化，同时改变运动皮层的振荡表现。然而，这些干扰可能并不局限于运动区域，其他部位如对神经发生和认知功能至关重要的海马体，可能在神经发生和振荡方面受到影响。为了解决SCI这一显著并发症，我们在SCI急性期评估了海马体的神经发生和节律。在本研究中，我们使用了40只雄性大鼠（假手术组W1 = 10只，SCI组W1 = 10只，假手术组W2 = 10只，SCI组W2 = 10只），研究结果显示，挫伤性SCI会降低海马体节律（δ波、θ波、β波、γ波）的功率和最高频率。此外，齿状回中双皮质素（DCX）阳性和5-溴脱氧尿嘧啶核苷（BrdU）阳性细胞显著减少；与节律功率下降显著相关。考虑到TUNEL检测分析，在受伤动物的海马体CA1区、CA3区和齿状回中有明显更多的凋亡细胞。此外，根据蛋白质免疫印迹分析，在神经发生和节律产生中起重要作用的受体（N-甲基-D-天冬氨酸受体、γ-氨基丁酸A型受体、M1型毒蕈碱受体）的表达在SCI后显著减弱。我们的研究表明，急性SCI会改变海马体节律的功率和最高频率，同时伴有海马体神经发生、细胞凋亡和受体表达的变化。
