Key Laboratory for Sustainable Development of Marine Fisheries, Ministry of Agriculture, Yellow Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Qingdao 266071, China; Laboratory for Marine Fisheries Science and Food Production Processes, Qingdao National Laboratory for Marine Science and Technology, Qingdao 266235, China; Shandong Key Laboratory of Marine Fisheries Biotechnology and Genetic Breeding, Yellow Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Qingdao 266071, China.
Key Laboratory for Sustainable Development of Marine Fisheries, Ministry of Agriculture, Yellow Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Qingdao 266071, China; Laboratory for Marine Fisheries Science and Food Production Processes, Qingdao National Laboratory for Marine Science and Technology, Qingdao 266235, China.
Comp Biochem Physiol Part D Genomics Proteomics. 2021 Sep;39:100833. doi: 10.1016/j.cbd.2021.100833. Epub 2021 Apr 7.
In addition to the typical sexual size dimorphism, considerable size differences within the female population of the Chinese tongue sole (Cynoglossus semilaevis) have become a further bottleneck of the improvement of sole aquaculture. To identify the internal mechanism, transcriptomic analysis and weighted gene co-expression network analysis (WGCNA) were employed simultaneously. Transcriptomic analyses of brain, pituitary gland, liver, gonad, and muscle tissues from two female groups with size differences identified 109, 698, 1325, 2299, and 2141 differentially expressed genes (DEGs), respectively. The results of these enrichment analyses suggest that the up-regulation of neuroactive ligand-receptor interaction, cell cycle, DNA replication, and MAPK signaling pathway in the group with larger females may be involved in the regulation of the observed growth differences. WGCNA of DEGs showed that cell cycle and DNA replication might be crucial pathways for accelerating cell growth in the groups with larger females. Finally, a series of hub genes including 6-phosphofructokinase type C (pfkp), ribosome biogenesis protein (wdr12), bleomycin hydrolase (blmh), and semaphorin-3A (sema3a) were recognized by the illustrated network map of modules. The linkage of cell cycle, DNA replication, and hub genes in the growth regulation of C. semilaevis provides further information for a better understanding of growth differences in fish.
除了典型的性二型外，中国舌鳎（Cynoglossus semilaevis）雌性群体中的显著体型差异已成为提高舌鳎养殖的又一瓶颈。为了鉴定其内在机制，我们同时进行了转录组分析和加权基因共表达网络分析（WGCNA）。对两组体型差异的雌性舌鳎的脑、垂体、肝、性腺和肌肉组织进行转录组分析，分别鉴定到 109、698、1325、2299 和 2141 个差异表达基因（DEGs）。这些富集分析的结果表明，体型较大的雌性群体中神经活性配体-受体相互作用、细胞周期、DNA 复制和 MAPK 信号通路的上调可能参与了观察到的生长差异的调控。DEGs 的 WGCNA 显示，细胞周期和 DNA 复制可能是体型较大的雌性群体中加速细胞生长的关键途径。最后，通过模块的图示网络图谱，识别到了一系列关键基因，包括 6-磷酸果糖激酶 C（pfkp）、核糖体生物发生蛋白（wdr12）、博来霉素水解酶（blmh）和 Sema3A（sema3a）。这些关键基因和细胞周期、DNA 复制在舌鳎生长调控中的联系，为进一步了解鱼类生长差异提供了更多信息。
