使用高效液相色谱法测定血清中性类固醇激素的自动化直接检测系统。

Automated direct assay system for the measurement of sex steroid hormones in serum using high-performance liquid chromatography.

作者信息

Suzuki Y, Hayashi N, Sekiba K

机构信息

Department of Obstetrics and Gynaecology, Okayama University Medical School, Japan.

出版信息

J Chromatogr. 1988 Apr 8;426(1):33-40. doi: 10.1016/s0378-4347(00)81924-6.

DOI:10.1016/s0378-4347(00)81924-6

PMID:3384879

Abstract

An automated direct assay system using high-performance liquid chromatography was developed for the simultaneous measurement of estradiol, estrone, progesterone, 17 alpha-hydroxyprogesterone, 20 alpha-hydroxyprogesterone, testosterone and androstenedione in biological fluids. A comparison between the values measured by this method and by radioimmunoassay revealed good correlation for estradiol (r = 0.938, p less than 0.001) and progesterone (r = 0.903, p less than 0.001). Estradiol and estrone could be analysed above the level of 250 pg/ml, and progesterone, 17 alpha-hydroxyprogesterone, 20 alpha-hydroxyprogesterone, testosterone and androstenedione could be analysed above the level of 5.0-7.5 ng/ml. The method was applied to the clinical appraisal of placental function and maturation of ovarian follicles.

摘要

开发了一种使用高效液相色谱的自动化直接检测系统，用于同时测量生物体液中的雌二醇、雌酮、孕酮、17α-羟孕酮、20α-羟孕酮、睾酮和雄烯二酮。该方法与放射免疫分析法测量值之间的比较显示，雌二醇（r = 0.938，p < 0.001）和孕酮（r = 0.903，p < 0.001）具有良好的相关性。雌二醇和雌酮可在250 pg/ml以上水平进行分析，孕酮、17α-羟孕酮、20α-羟孕酮、睾酮和雄烯二酮可在5.0 - 7.5 ng/ml以上水平进行分析。该方法应用于胎盘功能和卵泡成熟的临床评估。