Department of Bacteriology, ICMR-Regional Medical Research Centre (Dept. of Health Research, Ministry of Health & Family Welfare, Govt. of India), Chandrasekharpur, Bhubaneswar 751023, India.
Department of Life Sciences, Dibrugarh University, Dibrugarh 786004, Assam, India.
J Infect Public Health. 2021 May;14(5):628-637. doi: 10.1016/j.jiph.2021.02.007. Epub 2021 Feb 19.
The rapid emergence and variations of antibiotic resistance among common gram negative bacteria cause a significant concern specially in India and all over the world because of high mortality and morbidity rates.
In our study, we screened 189 bacterial isolates from Assam Medical College & Hospital, Dibrugarh for antibiotic resistance pattern and tried to identify the resistant genes causing responsible for β-lactam and fluoroquinolones resistance.
More than 80% and 45% strains were resistant to all the 3rd generation cephalosporins, fluoroquinolones respectively. Among the 3rd generation cephalosporin resistant strains, 38% and 24% isolates were only ESBL and MBL producers respectively and 11% were reported to have both ESBL and MBL genes. The ESBL positive isolates have shown the dominance of CTX-M3 gene. VIM-1 gene was mostly reported in MBL producers. Our study probably for the first time reporting SIM-1 and SPM-1 MBL gene from India. Mutations in QRDR is found to be the primary cause of fluoroquinolone resistance along with efflux pump and PMQR presence.
The study represents the first detailed study on antibiotic resistance from NE India this could help to take control measures for the emerging antibiotic resistance in hospital and community based infections in North East India.
常见革兰氏阴性菌对抗生素耐药性的迅速出现和变异引起了人们的高度关注，尤其是在印度和世界各地，因为其死亡率和发病率都很高。
在我们的研究中，我们从阿萨姆邦医学科学院和医院筛选了 189 株细菌分离株，以检测其抗生素耐药模式，并试图确定导致β-内酰胺类和氟喹诺酮类耐药的耐药基因。
超过 80%和 45%的菌株分别对所有第三代头孢菌素和氟喹诺酮类药物具有耐药性。在第三代头孢菌素耐药菌株中，38%和 24%的分离株分别仅为 ESBL 和 MBL 产生者，11%的分离株报告同时具有 ESBL 和 MBL 基因。ESBL 阳性分离株显示 CTX-M3 基因占优势。在 MBL 产生者中，主要报告了 VIM-1 基因。我们的研究可能首次从印度报告了 SIM-1 和 SPM-1 MBL 基因。QRDR 突变是氟喹诺酮类耐药的主要原因，同时还存在外排泵和 PMQR。
该研究代表了对印度东北部抗生素耐药性的首次详细研究，这有助于在印度东北部的医院和社区感染中采取控制新兴抗生素耐药性的措施。
