Kong Mengjuan, Wei Wei, Wang Weifeng, Chen Hongli, He Jiang
College of Chemistry and Chemical Engineering, Lanzhou University, Lanzhou 730000, PR China.
Lanzhou Institute of Chemical Physics, Chinese Academy of Sciences, Lanzhou 730000, PR China.
Spectrochim Acta A Mol Biomol Spectrosc. 2021 Aug 5;257:119773. doi: 10.1016/j.saa.2021.119773. Epub 2021 Apr 1.
It is found that MIL-100(Fe) gels, as a kind of metal-organic gels (MOGs), constitutting of iron (Fe) and trimesic acid (HBTC), has been regarded as the efficient catalyst of luminol chemiluminescence (CL) system without the presence of extra oxidants in the present work. MIL-100(Fe) gels that have possessed mimicking oxidase-like activity can excellently enhanced luminol CL intensity by accelerating the generation of reactive oxygen species. Furthermore, with the addition of uric acid (UA), the CL signal has been dramatically inhibited under alkaline condition. Hence, the CL intensity inhibiting ratio (I/I) was proportional to the increasing concentration of UA in the rang from 10 nM to 4000 nM with the detection limit of 5.9 nM. This method has been successfully applied for analysis of UA with acceptable recoveries ranging from 97.0% to 107.9% in urine sample. These results indicates that this study open up a novel, sensitive and convenient method to detect UA in biological samples.
研究发现，作为一种金属有机凝胶（MOG），由铁（Fe）和均苯三甲酸（HBTC）构成的MIL-100（Fe）凝胶在本工作中被视为在无额外氧化剂存在的情况下鲁米诺化学发光（CL）体系的高效催化剂。具有类似氧化酶活性的MIL-100（Fe）凝胶可通过加速活性氧的产生显著增强鲁米诺CL强度。此外，在碱性条件下，加入尿酸（UA）后，CL信号受到显著抑制。因此，在10 nM至4000 nM范围内，CL强度抑制率（I/I）与UA浓度的增加成正比，检测限为5.9 nM。该方法已成功应用于尿液样本中UA的分析，回收率在97.0%至107.9%之间，结果令人满意。这些结果表明，本研究开辟了一种检测生物样本中UA的新颖、灵敏且便捷的方法。
