FeOOH 纳米棒的类氧化酶特性增强的鲁米诺化学发光用于尿酸的灵敏检测。
Enhanced Luminol Chemiluminescence with Oxidase-like Properties of FeOOH Nanorods for the Sensitive Detection of Uric Acid.
机构信息
College of Chemistry and Bioengineering, Guilin University of Technology, Guilin 541004, China.
State Key Laboratory of Electroanalytical Chemistry, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun, Jilin 130022, China.
出版信息
Anal Chem. 2023 Feb 14;95(6):3267-3273. doi: 10.1021/acs.analchem.2c04247. Epub 2023 Feb 1.
FeOOH nanorods, as one-dimensional nanomaterials, have been widely used in many fields due to their stable properties, low cost, and easy synthesis, but their application in the field of chemiluminescence (CL) is rarely reported. In this work, FeOOH nanorods were synthesized by a simple and environmentally friendly one-pot hydrothermal method and used for the first time as a catalyst for generating strong CL with luminol without additional oxidant. Remarkably, luminol-FeOOH exhibits about 250 times stronger CL than the luminol-HO system. Its CL intensity was significantly quenched by uric acid. We established a simple, rapid, sensitive, and selective CL method for the detection of uric acid with a linear range of 20-1000 nM and a detection limit of 6.3 nM (S/N = 3). In addition, we successfully applied this method to the detection of uric acid in human serum, and the standard recoveries were 95.6-106.4%.
FeOOH 纳米棒作为一维纳米材料，由于其稳定的性质、低廉的成本和易于合成，已经在许多领域得到了广泛的应用，但在化学发光（CL）领域的应用却很少有报道。在这项工作中，我们通过一种简单、环保的一锅水热法合成了 FeOOH 纳米棒，并首次将其用作无需额外氧化剂即可产生强 CL 的催化剂与鲁米诺反应。值得注意的是，鲁米诺-FeOOH 产生的 CL 强度比鲁米诺-HO 体系强约 250 倍。其 CL 强度被尿酸显著猝灭。我们建立了一种简单、快速、灵敏、选择性的 CL 方法，用于检测尿酸，线性范围为 20-1000 nM，检测限为 6.3 nM（S/N = 3）。此外，我们成功地将该方法应用于人血清中尿酸的检测，标准回收率为 95.6-106.4%。
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