Department of Anesthesiology, Laiyang Central Hospital, Yantai City, China.
Neuroimmunomodulation. 2021;28(1):38-46. doi: 10.1159/000514548. Epub 2021 Apr 13.
The purpose of this study was to explore the role of curcumin (Cur) in isoflurane (ISO)-induced learning and memory dysfunction in Sprague-Dawley rats and further elucidate the mechanism of the protective effect produced by Cur.
Rat models of cognitive impairment were established by inhaling 3% ISO. The Morris water maze test was used to assess the cognitive function of rats. ELISA and qRT-PCR were used to analyze the protein levels of pro-inflammatory cytokines and expression levels of miR-181a-5p, respectively.
Cur significantly improved the ISO-induced cognitive dysfunction in rats and alleviated the ISO-induced neuroinflammation. miR-181a-5p was overexpressed in ISO-induced rats, while Cur treatment significantly reduced the expression of miR-181a-5p. Overexpression of miR-181a-5p promoted the cognitive impairment and the release of inflammatory cytokines and reversed the neuroprotective effect of Cur.
Cur has a protective effect on ISO-induced cognitive dysfunction, which may be achieved by regulating the expression of miR-181a-5p.
本研究旨在探讨姜黄素（Cur）在异氟醚（ISO）诱导的 Sprague-Dawley 大鼠学习记忆功能障碍中的作用，并进一步阐明 Cur 产生保护作用的机制。
通过吸入 3% ISO 建立认知障碍大鼠模型。采用 Morris 水迷宫试验评估大鼠的认知功能。ELISA 和 qRT-PCR 分别用于分析促炎细胞因子的蛋白水平和 miR-181a-5p 的表达水平。
Cur 显著改善了 ISO 诱导的大鼠认知功能障碍，并减轻了 ISO 诱导的神经炎症。ISO 诱导的大鼠中 miR-181a-5p 表达上调，而 Cur 处理显著降低了 miR-181a-5p 的表达。miR-181a-5p 的过表达促进了认知障碍和炎症细胞因子的释放，并逆转了 Cur 的神经保护作用。
Cur 对 ISO 诱导的认知功能障碍具有保护作用，其机制可能与调节 miR-181a-5p 的表达有关。
