Tian Xiang, Yuan Yawei, Wang Long
Department of Anesthesiology, Minda Hospital of Hubei Minzu University, Enshi, China.
Department of Anesthesiology, Shanghai First Maternity and Infant Hospital, School of Medicine, 12476Tongji University, Shanghai, China.
Hum Exp Toxicol. 2022 Jan-Dec;41:9603271221132152. doi: 10.1177/09603271221132152.
This observation aimed to investigate the effect of colorectal neoplasia differentially expressed (CRNDE) targeted miR-212-5p on cognitive impairment induced by isoflurane (ISO) anesthesia in rats.
The cognitive function of rats was measured by Morris water maze test. QRT-PCR detection of CRNDE and miR-212-5p expression levels in rats in each group. Double luciferase was used to verify the targeting relationship between miR-212-5p and CRNDE, and commercial kits were used to detect the level of inflammatory cytokines in hippocampus.
The concentration of CRNDE was enhanced in rats treated by ISO anesthetic. The neurological severity score was elevated, the escape latency of rats was prolonged, the stay time in the quadrant of the platform, and the number of times crossing the platform decreased in the ISO group. The above indexes of rats in ISO + si-CRNDE were improved. MiR-212-5p is a mediator in the management of CRNDE on cognition and inflammation.
CRNDE led to the deterioration of impairment on cognition induced by ISO through suppressing miR-212-5p expression and promoting neuroinflammation.
本观察旨在研究结直肠癌差异表达基因（CRNDE）靶向的miR-212-5p对异氟烷（ISO）麻醉诱导的大鼠认知功能障碍的影响。
采用Morris水迷宫试验测定大鼠的认知功能。通过QRT-PCR检测每组大鼠中CRNDE和miR-212-5p的表达水平。使用双荧光素酶验证miR-212-5p与CRNDE之间的靶向关系，并使用商业试剂盒检测海马中炎性细胞因子的水平。
ISO麻醉处理的大鼠中CRNDE浓度升高。ISO组大鼠的神经严重程度评分升高，逃避潜伏期延长，在平台象限的停留时间以及穿过平台的次数减少。ISO + si-CRNDE组大鼠的上述指标得到改善。MiR-212-5p是CRNDE对认知和炎症管理的介导因子。
CRNDE通过抑制miR-212-5p表达并促进神经炎症，导致ISO诱导的认知功能障碍恶化。
