生成具有少突胶质细胞和髓鞘的人类多能干细胞衍生的融合类器官。
Generation of human pluripotent stem cell-derived fused organoids with oligodendroglia and myelin.
机构信息
Department of Chemical and Biomolecular Engineering, Vanderbilt University, Nashville, TN 37212, USA.
Department of Cell Biology and Neuroscience, Rutgers University, Piscataway, NJ 08854, USA.
出版信息
STAR Protoc. 2021 Apr 7;2(2):100443. doi: 10.1016/j.xpro.2021.100443. eCollection 2021 Jun 18.
Current knowledge on brain oligodendrogenesis, composed of three sequential waves from different regions, is mainly obtained from rodent studies. Oligodendroglial development and myelination in the rodent brain may not fully mirror those processes in the human brain. Here, we provide a step-by-step protocol for generating fused forebrain organoids derived from human pluripotent stem cells. These fused organoids recapitulate human oligodendroglial developments, offering innovative insights into human myelination research . For complete details on the use and execution of this protocol, please refer to Cameron-Curry and Le Douarin (1995), Kessaris et al. (2006), and Kim et al. (2019).
目前关于脑少突胶质细胞发生的知识，由来自不同区域的三个连续波组成，主要来自于啮齿动物研究。啮齿动物大脑中的少突胶质细胞发育和髓鞘形成可能不完全反映人类大脑中的这些过程。在这里，我们提供了一个从人类多能干细胞生成融合前脑类器官的分步方案。这些融合类器官重现了人类少突胶质细胞的发育，为人类髓鞘形成研究提供了创新的见解。有关该方案使用和执行的完整详细信息，请参见 Cameron-Curry 和 Le Douarin（1995）、Kessaris 等人（2006）和 Kim 等人（2019）。
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