Department of Molecular Medicine & Pathology, University of Auckland, Auckland 1142, New Zealand.
Department of Developmental Biology, University of Pittsburgh, Pittsburgh, PA 15260, USA.
STAR Protoc. 2020 Oct 29;1(3):100150. doi: 10.1016/j.xpro.2020.100150. eCollection 2020 Dec 18.
Kidney organoids represent a physiologically advanced model for studying the mechanisms of kidney development and disease. Here, we describe a simple two-step protocol for the differentiation of human pluripotent stem cells into kidney organoids. Our approach involves suspension culture that allows for rapid and cost-effective bulk production of organoids, which is well suited for large-scale assays such as drug screening. The organoids correspond to fetal human kidney tissue and may be of limited use for modeling adult kidney function. For complete details on the use and execution of this protocol, please refer to Przepiorski et al. (2018).
肾类器官代表了一种更先进的生理模型，可用于研究肾脏发育和疾病的机制。在这里，我们描述了一种将人类多能干细胞分化为肾类器官的简单两步法方案。我们的方法涉及悬浮培养，可快速且经济高效地批量生产类器官，非常适合药物筛选等大规模检测。这些类器官与胎儿人类肾脏组织相对应，可能对模拟成人肾脏功能的用途有限。有关该方案使用和实施的完整详细信息，请参阅 Przepiorski 等人 (2018 年)。
