Institute of Microtechnology Technische Universität Braunschweig, Braunschweig, Germany.
Adv Biochem Eng Biotechnol. 2022;179:291-309. doi: 10.1007/10_2021_164.
Human health is threatened by the spread of antimicrobial resistance and resulting infections. One reason for the resistance spread is the treatment with inappropriate and ineffective antibiotics because standard antimicrobial susceptibility testing methods are time-consuming and laborious. To reduce the antimicrobial susceptibility detection time, minimize treatments with empirical broad-spectrum antibiotics, and thereby combat the further spread of antimicrobial resistance, faster and point-of-care methods are needed. This requires many different research approaches. Microfluidic systems for antimicrobial susceptibility testing offer the possibility to reduce the detection time, as small sample and reagent volumes can be used and the detection of single cells is possible. In some cases, the aim is to use human samples without pretreatment or pre-cultivation. This chapter first provides an overview of conventional detection methods. It then presents the potential of and various current approaches in microfluidics. The focus is on microfluidic methods for phenotypic antimicrobial susceptibility testing.
人类健康受到抗菌药物耐药性传播和由此产生的感染的威胁。耐药性传播的一个原因是使用不适当和无效的抗生素治疗，因为标准的抗菌药物敏感性测试方法既耗时又费力。为了减少抗菌药物敏感性检测时间，减少经验性广谱抗生素的使用，从而遏制抗菌药物耐药性的进一步传播，需要更快、更便于现场使用的方法。这需要许多不同的研究方法。用于抗菌药物敏感性测试的微流控系统提供了缩短检测时间的可能性，因为可以使用小样本和试剂体积，并且可以检测单细胞。在某些情况下，目标是使用未经预处理或预培养的人体样本。本章首先概述了传统的检测方法。然后介绍了微流控技术的潜力和各种当前方法。重点是用于表型抗菌药物敏感性测试的微流控方法。
