Guangdong Technology Research Center for Marine Algal Bioengineering, Guangdong Key Laboratory of Plant Epigenetics, College of Life Sciences and Oceanography, Shenzhen University, Shenzhen, 518055, People's Republic of China.
Shenzhen Engineering Laboratory for Marine Algal Biotechnology, Longhua Innovation Institute for Biotechnology, Shenzhen University, Shenzhen, 518055, People's Republic of China.
Ecotoxicology. 2021 Jul;30(5):975-985. doi: 10.1007/s10646-021-02407-4. Epub 2021 Apr 13.
Dinoflagellate blooms currently caused serious environmental problems in different areas of the world. Recent studies revealed close relationship between dinoflagellate blooms and microbial community dynamics, while less attention has been paid on the bacterial culturability change associated with the bloom. Here, we investigated the temporal variation of microbial community composition and culturability during the decline stage of an Akashiwo sanguinea bloom occurred in Shenzhen, China. The daily microbial community phylogenetic structures in water samples collected during a four-day period after the bloom peak were assessed through 16S rRNA gene amplicons sequencing on the MiSeq (Illumina) platform. The environmental parameters, Chlorophyll a concentrations, and total viable and culturable bacterial densities were also measured. Our results showed that Gamma-proteobacteria comprising mostly of Pseudoalteromonadaceae and Vibrionaceae was the predominant microbial class in the post-bloom samples, except for the second day. During that day, the represented groups switched to Alpha-proteobacteria (Rhizobiales) and Beta-proteobacteria (Comamonadaceae), with the microbial culturability decreased. Total viable bacterial densities reached the maximum value on the third day, with Gamma-proteobacteria regained the dominance till the fourth day. The dramatic microbial community succession and culturability variation observed in this study indicated the complication of algae-bacteria interactions during dinoflagellate bloom.
甲藻水华目前在世界不同地区造成了严重的环境问题。最近的研究表明，甲藻水华与微生物群落动态密切相关，而与水华相关的细菌可培养性变化则较少受到关注。在这里，我们研究了中国深圳一株夜光藻水华衰退阶段微生物群落组成和可培养性的时间变化。通过 MiSeq（Illumina）平台对水华高峰期后四天内采集的水样中的 16S rRNA 基因扩增子进行测序，评估了每日微生物群落系统发育结构。还测量了环境参数、叶绿素 a 浓度以及总活菌和可培养细菌的密度。我们的结果表明，除了第二天外，在水华后的样品中，Gamma-变形菌（主要由假交替单胞菌科和弧菌科组成）是主要的微生物类群。在那一天，代表群体转变为 Alpha-变形菌（根瘤菌目）和 Beta-变形菌（丛毛单胞菌科），同时微生物可培养性下降。总活菌密度在第三天达到最大值，第四天 Gamma-变形菌重新占据优势。本研究中观察到的微生物群落演替和可培养性的剧烈变化表明，在甲藻水华期间，藻类-细菌相互作用的复杂性。
