基于组织芯片的滑膜肉瘤及其组织学模拟物中TLE1的免疫组织化学研究

Tissue microarray based immunohistochemical study of TLE1 in synovial sarcoma and its histologic mimics.

作者信息

Sivakoti Sumitra, Uppin Shantveer G, Uppin Megha S, Tara Roshini P, Prayaga Aruna K, Challa Sundaram

机构信息

Department of Pathology, Nizam's Institute of Medical Sciences, Hyderabad; Department of Pathology and Lab Medicine, All India Institute of Medical Sciences, Bibinagar, Telangana, India.

Department of Pathology, Nizam's Institute of Medical Sciences, Hyderabad, India.

出版信息

Indian J Pathol Microbiol. 2021 Apr-Jun;64(2):310-315. doi: 10.4103/IJPM.IJPM_425_20.

DOI:10.4103/IJPM.IJPM_425_20

PMID:33851625

Abstract

BACKGROUND AND AIMS

Molecular analysis is gold standard for diagnosis of synovial sarcoma (SS) but use of these ancillary techniques is limited by many practical issues like cost and limited resources. Several studies analyzed TLE1 as a diagnostic immunohistochemical marker for synovial sarcoma and few studies disagreed. The objective of the study was to evaluate immunohistochemical expression of TLE1 in synovial sarcoma and its histological mimics.

METHODS

The study included a total of 63 cases; of which 28 were synovial sarcomas (SS) and 35 its histologic mimics. A tissue microarray was constructed from these cases and subjected to TLE immunostaining. Nuclear immunoreactivity of TLE1 was graded as 0, 1+, 2+ and 3+ based on intensity and percentage of cells.

RESULTS

All SS except one (27/28; 96.4%) were positive for TLE 1. These included 18 of monophasic spindle cell type (94.7%), 5 biphasic type (100%), followed by two each (100%) of poorly differentiated and calcifying type of SS. Of the other tumours 2 GISTs (50%), 2 haemangiopericytoma (66.7%), 2 schwannomas (50%) and one mesenchymal chondrosarcoma (33.3%) were positive for TLE1.

CONCLUSION

TLE 1 is a highly sensitive marker with reasonable specificity for synovial sarcoma. Awareness of TLE1 expression in other tumours, is important to avoid misdiagnosis.

摘要

背景与目的

分子分析是滑膜肉瘤（SS）诊断的金标准，但这些辅助技术的应用受到成本和资源有限等诸多实际问题的限制。多项研究分析了TLE1作为滑膜肉瘤的诊断性免疫组化标志物，少数研究持不同意见。本研究的目的是评估TLE1在滑膜肉瘤及其组织学相似肿瘤中的免疫组化表达。

方法

本研究共纳入63例病例；其中28例为滑膜肉瘤（SS），35例为其组织学相似肿瘤。从这些病例中构建组织微阵列，并进行TLE免疫染色。根据细胞强度和百分比，将TLE1的核免疫反应性分为0、1+、2+和3+。

结果

除1例（27/28；96.4%）外，所有滑膜肉瘤TLE 1均呈阳性。其中单相梭形细胞型18例（94.7%），双相型5例（100%），低分化型和钙化型滑膜肉瘤各2例（100%）。在其他肿瘤中，2例胃肠道间质瘤（GISTs，50%）、2例血管外皮细胞瘤（66.7%）、2例神经鞘瘤（50%）和1例间叶性软骨肉瘤（33.3%）TLE1呈阳性。