Dept. of Pathology, Brigham and Women's Hospital, 75 Francis St., Boston, MA 02115, USA.
Am J Clin Pathol. 2011 Jun;135(6):839-44. doi: 10.1309/AJCP45SSNAOPXYXU.
Transducer-like enhancer of split 1 (TLE1) is overexpressed in synovial sarcomas. We investigated TLE1 expression by immunohistochemical analysis in a well-characterized series of synovial sarcomas and other mesenchymal tumors most commonly considered in the differential diagnosis. Whole tissue sections of 212 tumors were evaluated: 73 synovial sarcomas (23 biphasic, 28 monophasic, 22 poorly differentiated), 47 malignant peripheral nerve sheath tumors (MPNSTs), 49 solitary fibrous tumors (SFTs), 20 fibrosarcomatous variants of dermatofibrosarcoma protuberans, and 23 Ewing sarcomas/primitive neuroectodermal tumors (PNETs). All monophasic and poorly differentiated SSs and Ewing sarcoma/PNETs were previously confirmed to harbor t(X;18) and EWSR1 gene rearrangements, respectively. In total, 60 (82%) of 73 synovial sarcomas were positive for TLE1, including 18 biphasic (78%), 22 monophasic (79%), and 20 poorly differentiated (91%) tumors. Of the other tumors, only 7 MPNSTs (15%) and 4 SFTs (8%) were positive for TLE1, most of which showed only weak staining. TLE1 is a sensitive and specific marker for synovial sarcoma and can be helpful to distinguish synovial sarcoma from histologic mimics, particularly if moderate or strong staining is observed. In this study, only a small subset of MPNSTs and SFTs showed limited staining for TLE1.
转录因子样增强子 1（TLE1）在滑膜肉瘤中过表达。我们通过免疫组织化学分析对一组特征明确的滑膜肉瘤和其他间叶性肿瘤进行了 TLE1 表达研究，这些肿瘤是滑膜肉瘤鉴别诊断中最常考虑的。对 212 个肿瘤的全组织切片进行了评估：73 个滑膜肉瘤（23 个双相型、28 个单相型、22 个低分化型）、47 个恶性外周神经鞘瘤（MPNSTs）、49 个孤立性纤维瘤（SFTs）、20 个隆突性皮肤纤维肉瘤的纤维肉瘤样变体，以及 23 个尤文肉瘤/原始神经外胚层肿瘤（PNETs）。所有单相和低分化型 SS 以及尤文肉瘤/PNET 均经证实分别存在 t(X;18)和 EWSR1 基因重排。共有 60 个（82%）滑膜肉瘤为 TLE1 阳性，包括 18 个双相型（78%）、22 个单相型（79%）和 20 个低分化型（91%）肿瘤。其他肿瘤中，只有 7 个 MPNSTs（15%）和 4 个 SFTs（8%）为 TLE1 阳性，其中大多数仅显示弱阳性染色。TLE1 是滑膜肉瘤的一种敏感且特异的标志物，有助于将滑膜肉瘤与组织学模拟物区分开来，特别是如果观察到中等或强染色时。在本研究中，只有一小部分 MPNSTs 和 SFTs 显示出对 TLE1 的有限染色。
