Institute for Public Health Vojvodina, Novi Sad, Serbia.
Faculty of Medicine, University of Novi Sad, Novi Sad, Serbia.
PLoS One. 2021 Apr 14;16(4):e0249134. doi: 10.1371/journal.pone.0249134. eCollection 2021.
This study evaluates the pre-vaccination prevalence of HPV infection in women from Vojvodina, Serbia, according to age and cytological status. A total of 1,495 women, ranging from 18 to 65 years of age, with different cytological results were enrolled. The HPV genotyping assay was performed using the EUROArray HPV test in order to detect thirty genitally relevant HPV subtypes. In our study, the most prevalent genotypeswere HPV 16, 31, 51, and 53. Among these, HPV 16 was consistently present in all cytological subgroups. Twelve HPV genotypes classified as carcinogenic to humans (Group 1) were detected in 77.8.0% of HSIL/ASCH and 55.0% of NILM with abnormal colposcopy findings. Six possible carcinogens-HRs (group 2B) were often found in women with normal cytology (14.8%) and mild abnormalities (ASCUS and LSIL), but with lower frequence in HSIL/ASCH lesions (7.1%). HPVs 6 and 11(Group 3) were not found in the cases of HSIL/ASCH. Unclassified HPV types were equally distributed in all cytology groups: 20.7%, 19.1%, 16.3% and 13% of NILM, ASCUS, LSIL and HSIL/ASCH, respectively. Our findings highlight that majority of abnormal Pap test results are caused by Group 1 HPVs among women from our region. Low frequency HPVs of group 2A/2B, especially HSIL/ASCH, supports the conclusion that individual genotypes require consideration of each type as an individual agent. We expect a positive impact of HPV vaccine in reducing HPV-associated cervical lesions among women from Vojvodina province, after establishing vaccination programs in our country.
本研究评估了塞尔维亚伏伊伏丁那地区女性的 HPV 感染流行率，根据年龄和细胞学状态进行分析。共纳入了 1495 名年龄在 18 至 65 岁之间的女性，她们的细胞学结果不同。使用 EUROArray HPV 检测方法进行 HPV 基因分型检测，以检测三十种与生殖器相关的 HPV 亚型。在我们的研究中，最常见的基因型是 HPV 16、31、51 和 53。其中，HPV 16 始终存在于所有细胞学亚组中。12 种被归类为人类致癌的 HPV 基因型（第 1 组）在所有 HSIL/ASCH 中占 77.8.0%，在所有异常阴道镜检查结果的 NILM 中占 55.0%。6 种可能致癌的 HPV-HR（第 2B 组）在细胞学正常（14.8%）和轻度异常（ASCUS 和 LSIL）的女性中经常发现，但在 HSIL/ASCH 病变中频率较低（7.1%）。HPV 6 和 11（第 3 组）在 HSIL/ASCH 病例中未发现。未分类的 HPV 类型在所有细胞学组中分布均匀：NILM、ASCUS、LSIL 和 HSIL/ASCH 中的分别为 20.7%、19.1%、16.3%和 13%。我们的研究结果表明，在我们地区的女性中，大多数异常巴氏试验结果是由第 1 组 HPV 引起的。HPV 2A/2B 组的低频率，特别是在 HSIL/ASCH 中，支持这样的结论，即个体基因型需要考虑每种类型作为一个单独的作用物。我们期望 HPV 疫苗的使用在建立疫苗接种计划后，会降低伏伊伏丁那地区女性中 HPV 相关的宫颈病变。
