用于宫颈样本基因分型的Anyplex II HPV28和Euroarray HPV的分析性能评估
Analytical performance evaluation of Anyplex II HPV28 and Euroarray HPV for genotyping of cervical samples.
作者信息
Latsuzbaia Ardashel, Tapp Jessica, Nguyen Trung, Fischer Marc, Arbyn Marc, Weyers Steven, Mossong Joël
机构信息
Surveillance & Epidemiology of Infectious Diseases, Laboratoire National de Santé, Dudelange, Luxembourg.
Surveillance & Epidemiology of Infectious Diseases, Laboratoire National de Santé, Dudelange, Luxembourg.
出版信息
Diagn Microbiol Infect Dis. 2016 Jul;85(3):318-322. doi: 10.1016/j.diagmicrobio.2016.04.011. Epub 2016 Apr 19.
Analytically accurate human papillomavirus (HPV) genotyping methods are required to assess the impact of HPV vaccination. The aim of this study was to evaluate the analytical performance of Anyplex II HPV28 (Seegene, Korea) and Euroarray HPV (Euroimmun, Germany) genotyping kits, for conducting a future HPV vaccine efficacy monitoring study in Luxembourg. A total number of 150 cervical swabs were collected from women with mean age 31.4 years. Agreements for detecting any HPV between Aptima/Anyplex (88.0%) and Aptima/Euroarray (90.7%) were similar. Agreement of Anyplex/EuroArray with Aptima was higher for Genotypes 16, 18 or 45 than for the other 11 HPVs. The average number of HPV genotypes detected per sample was similar with 2.6 and 2.5, for Anyplex and EuroArray, respectively. In conclusion, Anyplex and Euroarray showed high agreement in general and in particular for detecting genotypes contained in HPV vaccines.
为评估人乳头瘤病毒（HPV）疫苗接种的影响，需要分析准确的HPV基因分型方法。本研究的目的是评估Anyplex II HPV28（韩国Seegene公司）和Euroarray HPV（德国Euroimmun公司）基因分型试剂盒的分析性能，以便在卢森堡开展未来的HPV疫苗效力监测研究。共收集了150份宫颈拭子，来自平均年龄为31.4岁的女性。Aptima/Anyplex（88.0%）和Aptima/Euroarray（90.7%）之间检测任何HPV的一致性相似。对于16、18或45型，Anyplex/EuroArray与Aptima的一致性高于其他11种HPV。Anyplex和EuroArray每个样本检测到的HPV基因型平均数量相似，分别为2.6和2.5。总之，Anyplex和Euroarray总体上一致性较高，尤其是在检测HPV疫苗所含基因型方面。
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