一项关于定制高频不可逆电穿孔发生器在不同组织中产生临床相关消融区域的研究。

An study of a custom-made high-frequency irreversible electroporation generator on different tissues for clinically relevant ablation zones.

机构信息

Energy-based Tumor Ablation Laboratory, School of Mechatronic Engineering and Automation, Shanghai University, Shanghai, China.

Department of Surgery, University of Saskatchewan, Saskatoon, Canada.

出版信息

Int J Hyperthermia. 2021;38(1):593-603. doi: 10.1080/02656736.2021.1912417.

DOI:10.1080/02656736.2021.1912417

PMID:33853496

Abstract

PURPOSE

To examine the ablation zone, muscle contractions, and temperature increases in both rabbit liver and kidney models for a custom-made high-frequency irreversible electroporation (H-FIRE) generator.

MATERIALS AND METHODS

A total of 18 New Zealand white rabbits were used to investigate five H-FIRE protocols ( = 3 for each protocol) and an IRE protocol ( = 3) for the performance of the designed H-FIRE device in both liver and kidney tissues. The ablation zone was determined by using histological analysis 72 h after treatment. The extent of muscle contractions and temperature change during the application of pulse energy were measured by a commercial accelerometer attached to animals and fiber optic temperature probe inserted into organs with IRE electrodes, respectively.

RESULTS

All H-FIRE protocols were able to generate visible ablation zones without muscle contractions, for both liver and kidney tissues. The area of ablation zone generated in H-FIRE pulse protocols (e.g., 0.3-1 μs, 2000 V, and 90-195 bursts) appears similar to that of IRE protocol (100 μs, 1000 V, and 90 pulses) in both liver and kidney tissues. No significant temperature increase was noticed except for the protocol with the highest pulse energy (e.g., 1 μs, 2000 V, and 180 bursts).

CONCLUSION

Our work serves to complement the current H-FIRE pulse waveforms, which can be optimized to significantly improve the quality of ablation zone in terms of precision for liver and kidney tumors in clinical setting.

摘要

目的

研究定制高频不可逆电穿孔（H-FIRE）发生器在兔肝、肾模型中的消融区、肌肉收缩和温度升高情况。

材料与方法

共使用 18 只新西兰白兔，分别对 5 种 H-FIRE 方案（每种方案 3 只）和 1 种 IRE 方案（3 只）进行研究，以评估设计的 H-FIRE 设备在肝、肾组织中的性能。治疗后 72 小时，通过组织学分析确定消融区。使用商业加速度计测量脉冲能量应用过程中肌肉收缩的程度，使用插入带 IRE 电极的器官的光纤温度探头测量温度变化。

结果

所有 H-FIRE 方案均能在肝、肾组织中产生无肌肉收缩的可见消融区。H-FIRE 脉冲方案（如 0.3-1 μs、2000 V 和 90-195 次脉冲）生成的消融区面积与 IRE 方案（100 μs、1000 V 和 90 次脉冲）相似。除了能量最高的方案（如 1 μs、2000 V 和 180 次脉冲）外，未观察到明显的温度升高。