Brewton R G, MacCabe J A
Department of Zoology, University of Tennessee, Knoxville 37996-0810.
J Exp Zool. 1988 Apr;246(1):103-7. doi: 10.1002/jez.1402460113.
Excised tissues from the prospective posterior necrotic zone (pPNZ) of the stage 21 chick wing were cultured in the presence of the calmodulin antagonists/protein kinase C inhibitors trifluoperazine (TFP) or chlorpromazine (CPZ). The appearance of cell death in vitro was not affected by the drugs. Macrophages differentiated normally and were competent to engulf debris. Lysosomal fusion with phagosomes and the digestion of most debris also occurred in the presence of the drugs. However, the macrophages were unable to process internalized cell membranes properly and continued accumulating membrane until they were grossly distended. The effect was reversible upon removal of the drugs. The results suggest a role for calmodulin and/or protein kinase C in the proper recycling of internalized membrane in embryonic macrophages.
从21期鸡胚翅膀预期的后坏死区（pPNZ）切除的组织，在钙调蛋白拮抗剂/蛋白激酶C抑制剂三氟拉嗪（TFP）或氯丙嗪（CPZ）存在的情况下进行培养。体外细胞死亡的出现不受这些药物影响。巨噬细胞正常分化并能够吞噬碎片。在药物存在的情况下，溶酶体也会与吞噬体融合并消化大部分碎片。然而，巨噬细胞无法正常处理内化的细胞膜，并且持续积累膜，直到它们严重膨胀。去除药物后，这种效应是可逆的。结果表明钙调蛋白和/或蛋白激酶C在胚胎巨噬细胞内化膜的正常再循环中发挥作用。
