钙调蛋白拮抗剂可抑制培养的库普弗细胞的吞噬活性。

Calmodulin antagonists inhibit the phagocytic activity of cultured Kupffer cells.

作者信息

Watanabe S, Hirose M, Miyazaki A, Tomono M, Takeuchi M, Kitamura T, Namihisa T

机构信息

Department of Gastroenterology, Juntendo University School of Medicine, Tokyo, Japan.

出版信息

Lab Invest. 1988 Aug;59(2):214-8.

PMID:3404973

Abstract

The mechanism of phagocytosis by Kupffer cells is still unknown. In this study we found that trifluoperazine, chlorpromazine, and W-7, drugs which bind to Ca2+-calmodulin and inhibit its interaction with other proteins, inhibit phagocytosis by cultured Kupffer cells using polystyrene beads, time-lapse VTR systems, and fluorescent staining techniques. Inhibitory effects of these drugs on phagocytosis suggests that the Ca2+-calmodulin system may be involved in this complex cellular function and the integrity of the cytoskeletal system of Kupffer cells is essential to this phenomenon.

摘要

库普弗细胞的吞噬作用机制仍然未知。在本研究中，我们发现三氟拉嗪、氯丙嗪和W-7（这些药物可与钙调蛋白结合并抑制其与其他蛋白质的相互作用），使用聚苯乙烯微球、延时录像系统和荧光染色技术，抑制培养的库普弗细胞的吞噬作用。这些药物对吞噬作用的抑制作用表明，钙调蛋白系统可能参与了这一复杂的细胞功能，并且库普弗细胞细胞骨架系统的完整性对此现象至关重要。