LeBelle M, Lauriault G, Callahan S, Latham D, Chiarelli C, Beckstead H
Beureau of Drug Research, Health Protection Branch, Tunney's Pasture, Ottawa, ON, Canada.
J Forensic Sci. 1988 May;33(3):662-75.
A laboratory system of examination of illicit cocaine exhibits is described. Separation and identification of many of the components in exhibits are achieved by the use of capillary column gas chromatography and a Finnigan ion trap detector. Further examination and quantitation of the components of exhibits is achieved using two high performance liquid chromatographic (HPLC) systems. Both of these systems use identical reverse phase C8 columns. System 1 employs a solvent composed of 40% acetonitrile, 10% tetrahydrofuran and 50% 0.1% v/v aqueous triethylamine. The eluant is monitored at 280 nm. This system is preferred for routine quantitative analysis of cocaine and related alkaloids in exhibits. System 2 employs a solvent composed of 30% acetonitrile and 70% 0.05M phosphate buffer (pH = 5.0). The eluant from this system is monitored at both 220 and 280 nm. This system offers advantages in sensitivity. The relative retention times of a number of relevant substances as determined with gas chromatography and the two HPLC systems are given. The utility of the methodology for the identification and comparison of exhibits is demonstrated.
描述了一种用于检验非法可卡因展品的实验室系统。通过使用毛细管柱气相色谱法和菲尼根离子阱检测器，可实现展品中多种成分的分离和鉴定。使用两个高效液相色谱（HPLC）系统对展品成分进行进一步检验和定量。这两个系统均使用相同的反相C8柱。系统1采用由40%乙腈、10%四氢呋喃和50% 0.1%（v/v）三乙胺水溶液组成的溶剂。洗脱液在280 nm处进行监测。该系统适用于展品中可卡因及相关生物碱的常规定量分析。系统2采用由30%乙腈和70% 0.05M磷酸盐缓冲液（pH = 5.0）组成的溶剂。该系统的洗脱液在220和280 nm处均进行监测。该系统在灵敏度方面具有优势。给出了通过气相色谱法和两个HPLC系统测定的多种相关物质的相对保留时间。证明了该方法在展品鉴定和比较中的实用性。
