一种用于证据材料酯酶D亚型分型的混合两性电解质聚焦技术。
A hybrid ampholyte focusing technique for esterase D subtyping of evidentiary material.
作者信息
Budowle B, Gambel A M
机构信息
Forensic Science Research and Training Section, FBI Academy, Quantico, VA.
出版信息
J Forensic Sci. 1988 May;33(3):738-43.
An ultrathin-layer polyacrylamide gel isoelectric focusing technique that uses a composite of ampholytes from three commercial sources is described for subtyping esterase D. All common allelic products of esterase D were separated clearly. The technique described in this paper provides a higher conclusive call rate on known blood specimens (95.8%) and questioned bloodstains (69.7%) compared with continuous zone electrophoresis in agarose gels (89.9 and 37.6%, respectively).
描述了一种超薄层聚丙烯酰胺凝胶等电聚焦技术，该技术使用来自三种商业来源的两性电解质复合物对酯酶D进行亚型分析。酯酶D的所有常见等位基因产物都被清晰分离。与琼脂糖凝胶连续区带电泳相比（分别为89.9%和37.6%），本文所述技术对已知血样（95.8%）和可疑血迹（69.7%）的确定检出率更高。
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