新鲜血液和血痕中酯酶D及磷酸葡萄糖变位酶亚型的同步电泳分析
The simultaneous electrophoretic analysis of esterase D and phosphoglucomutase subtyping in fresh blood and in dried bloodstains.
作者信息
Adamo R, Kobilinksy L
出版信息
J Forensic Sci. 1984 Apr;29(2):436-44.
PMID:6233389
Abstract
Phosphoglucomutase1 (PGM) subtyping and esterase D phenotyping were simultaneously performed by electrophoresis of bloodstained fibers using agarose and a Tris-maleic acid buffer system , pH 5.4. This method reduces anodal gel shrinkage and shortens development time when compared to the conventional electrophoretic technique for PGM subtyping which is performed at pH 7.4 using an agarose-starch substrate.
摘要
采用琼脂糖和pH 5.4的Tris-马来酸缓冲系统，通过对血渍纤维进行电泳，同时进行磷酸葡萄糖变位酶1（PGM）亚型分析和酯酶D表型分析。与使用琼脂糖-淀粉底物在pH 7.4下进行PGM亚型分析的传统电泳技术相比，该方法减少了阳极凝胶收缩并缩短了显影时间。
相似文献
1
2
The simultaneous separation of the enzymes glyoxalase I, esterase D, and phosphoglucomutase.乙二醛酶I、酯酶D和磷酸葡萄糖变位酶的同时分离。
J Forensic Sci. 1986 Oct;31(4):1439-49.
3
Simultaneous phenotyping of erythrocyte acid phosphatase and esterase D by nonequilibrium agarose isoelectric focusing.通过非平衡琼脂糖等电聚焦对红细胞酸性磷酸酶和酯酶D进行同步表型分析。
Electrophoresis. 1989 Oct;10(10):718-21. doi: 10.1002/elps.1150101011.
4
An agarose gel electrophoretic method for typing phosphoglucomutase-1, esterase D, or glyoxalase I.一种用于磷酸葡萄糖变位酶-1、酯酶D或乙二醛酶I分型的琼脂糖凝胶电泳方法。
J Forensic Sci. 1985 Oct;30(4):1216-20.
5
Non-equilibrium pH gradient electrophoresis (NEPHGE) on ultrathin polyacrylamide gels containing separators: improved erythrocyte phosphoglucomutase (PGM) and esterase D (EsD) diagnosis in red cell lysates and bloodstains.在含有隔板的超薄聚丙烯酰胺凝胶上进行非平衡pH梯度电泳（NEPHGE）：改进红细胞裂解物和血迹中红细胞磷酸葡萄糖变位酶（PGM）和酯酶D（EsD）的诊断。
Forensic Sci Int. 1989 Jul;42(1-2):43-50. doi: 10.1016/0379-0738(89)90197-7.
6
7
A hybrid ampholyte focusing technique for esterase D subtyping of evidentiary material.一种用于证据材料酯酶D亚型分型的混合两性电解质聚焦技术。
J Forensic Sci. 1988 May;33(3):738-43.
8
Discussion of "The simultaneous separation of the enzymes glyoxalase I, esterase D, and phosphoglucomutase".《乙二醛酶I、酯酶D和磷酸葡萄糖变位酶的同步分离》的讨论
J Forensic Sci. 1987 Nov;32(6):1498-500.
9
10
Simultaneous subtyping of group specific component and esterase D by isoelectric focusing on agarose gels.通过在琼脂糖凝胶上进行等电聚焦同时对群特异性成分和酯酶D进行亚型分析。
J Forensic Sci. 1993 Nov;38(6):1488-90.
引用本文的文献
1
Simultaneous phenotyping of genetic markers for paternity testing.用于亲子鉴定的遗传标记的同步表型分析。
Z Rechtsmed. 1987;99(2):135-42. doi: 10.1007/BF00200633.
Zotero 插件