Zhang Wei, Wang Huaying, Dong Jianhua, Zhang Tengjiao, Xiao Hongxing
Key Laboratory of Molecular Epigenetics of Ministry of Education Northeast Normal University Changchun 130024 China.
Appl Plant Sci. 2021 Mar 16;9(3):e11412. doi: 10.1002/aps3.11412. eCollection 2021 Mar.
is an ideal taxon for studying the evolution of adaptive radiation. Current phylogenies of based on different molecular markers are inconsistent, and therefore a clear and accurate phylogeny remains uncertain. Analyzing the chloroplast genome, with its simple structure and low recombination rate, may help solve this problem.
Next-generation sequencing data were generated or downloaded for species, enabling their chloroplast genomes to be assembled. The assemblies were used to estimate the genome characteristics and infer the phylogeny of .
In this study, chloroplast genome sequences were assembled for species distributed across Asia, North America, and Europe. Three of the genes analyzed (, , and ) were shown to be under positive selection and may be related to adaptation. The phylogenetic tree of showed that its member species formed two clades with high support, North American and European species, with the Asian species being paraphyletic; and clustered with the North American species, while the remaining Asian species were found in the European clade. In addition, var. should be considered as a separate species rather than a variety.
The complete chloroplast genomes of these species provide new insights into the reconstruction of the phylogeny of related species and contribute to the further study of this genus.
是研究适应性辐射进化的理想分类单元。目前基于不同分子标记的系统发育树不一致，因此清晰准确的系统发育关系仍不确定。分析叶绿体基因组，因其结构简单且重组率低，可能有助于解决这一问题。
为 个物种生成或下载了二代测序数据，从而能够组装它们的叶绿体基因组。这些组装结果用于估计基因组特征并推断 的系统发育关系。
在本研究中，为分布于亚洲、北美和欧洲的 个物种组装了叶绿体基因组序列。分析的三个基因（ 、 和 ）显示受到正选择，可能与适应性有关。 的系统发育树表明其成员物种形成了两个得到高度支持的分支，北美和欧洲物种，亚洲物种为并系群； 和 与北美物种聚类，而其余亚洲物种则位于欧洲分支中。此外，变种 应被视为一个独立的物种而非变种。
这 个物种的完整叶绿体基因组为相关物种系统发育的重建提供了新见解，并有助于对该属的进一步研究。
