Faviana Pinuccia, Boldrini Laura, Erba Paola Anna, Di Stefano Iosè, Manassero Francesca, Bartoletti Riccardo, Galli Luca, Gentile Carlo, Bardi Massimo
Department of Surgical, Medical, Molecular Pathology and Critical Area, University of Pisa, Pisa, Italy.
Department of Translational Research and New Technologies in Medicine and Surgery, University of Pisa, Pisa, Italy.
Front Oncol. 2021 Mar 29;11:650249. doi: 10.3389/fonc.2021.650249. eCollection 2021.
The aim of the present study was to evaluate whether prostate cancer (PC) patients can be accurately classified on the bases of tissue expression of gastrin-releasing peptide receptor (GRPR) and prostate-specific membrane antigen (PSMA). This retrospective study included 28 patients with PC. Formalin-fixed paraffin-embedded samples were used for diagnosis. Immunohistochemistry staining techniques were used to evaluate PSMA and GRPR expression (both number of cells expressed and % of area stained). To assess the independent associations among selected variables, a multi-dimensional scaling (MDS) analysis was used. It was found that the PSMA expression was inversely correlated with GRPR expression. Only the number of cells expressing GRPR was significantly related to the Gleason score. Both the percentage of area expressing GRPR and the number of cells expressing PSMA were close to reaching significance at the 0.05 level. MDS provided a map of the overall, independent association confirming that GRPR and PSMA represent inversely correlated measures of the same dimension. In conclusion, our data showed that GRPR expression should be evaluated in prostate biopsy specimens to improve our ability to detect PC with low grades at the earliest phases of development. Considering that GRPRs appear to be directly involved in the mechanisms of tumor proliferation, advancements in nuclear medicine radiotherapy can focus on this receptor to improve the therapeutic approach to PC. Further studies in our laboratory will investigate the molecular mechanisms of activation based on GRPR.
本研究的目的是评估前列腺癌（PC）患者是否能够基于胃泌素释放肽受体（GRPR）和前列腺特异性膜抗原（PSMA）的组织表达进行准确分类。这项回顾性研究纳入了28例PC患者。采用福尔马林固定石蜡包埋样本进行诊断。免疫组织化学染色技术用于评估PSMA和GRPR的表达（包括表达的细胞数量和染色面积百分比）。为了评估选定变量之间的独立关联，使用了多维标度（MDS）分析。结果发现，PSMA表达与GRPR表达呈负相关。只有表达GRPR的细胞数量与Gleason评分显著相关。表达GRPR的面积百分比和表达PSMA的细胞数量在0.05水平上均接近具有统计学意义。MDS提供了一个整体独立关联的图谱，证实GRPR和PSMA代表同一维度的负相关指标。总之，我们的数据表明，应在前列腺活检标本中评估GRPR表达，以提高我们在PC发展的最早阶段检测低级别PC的能力。鉴于GRPR似乎直接参与肿瘤增殖机制，核医学放疗的进展可以聚焦于该受体，以改善PC的治疗方法。我们实验室的进一步研究将探讨基于GRPR的激活分子机制。
