Department of Hematology, Children's Hospital of Soochow University, Suzhou, Jiangsu Province, China.
Department of Hematology, Anhui Provincial Children's Hospital, Anhui Province, China.
Biomed Res Int. 2021 Mar 27;2021:6614784. doi: 10.1155/2021/6614784. eCollection 2021.
To explore the immune cell therapy for T cell lymphoma, we developed CD4-specific chimeric antigen receptor- (CAR-) engineered T cells (CD4CART), and the cytotoxic effects of CD4CART cells were determined in vitro and in vivo.
CD4CART cells were obtained by transduction of lentiviral vector encoding a single-chain antibody fragment (scFv) specific for CD4 antigen, costimulatory factor CD28 fragment, and intracellular signal transduction domain of CD3 fragments. Control T cells were obtained by transduction of reporter lentiviral vector. The cytotoxicity, tumor growth, and survival rate of mice with T cell lymphoma were analyzed after adoptive T cell transfer in vivo.
CD4CART cells had potent cytotoxic activity against CD4+ T1301 tumor T cells in a concentration-dependent manner. In addition, adoptive CD4CART cell transfer significantly suppressed tumor growth and improved animal survival with T cell lymphoma, compared to the mice who received control T cells and PBS.
CD4CART cells have potent cytotoxic effects on T cell lymphoma. The study provided an experimental basis for CD4CART-mediated therapy of T cell lymphoma.
为了探索 T 细胞淋巴瘤的免疫细胞治疗，我们开发了 CD4 特异性嵌合抗原受体-（CAR-）工程 T 细胞（CD4CART），并在体外和体内测定了 CD4CART 细胞的细胞毒性作用。
通过转导编码针对 CD4 抗原、共刺激因子 CD28 片段和 CD3 片段胞内信号转导域的单链抗体片段（scFv）的慢病毒载体获得 CD4CART 细胞。通过转导报告基因慢病毒载体获得对照 T 细胞。分析体内过继转移 T 细胞后 T 细胞淋巴瘤小鼠的细胞毒性、肿瘤生长和存活率。
CD4CART 细胞以浓度依赖的方式对 CD4+T1301 肿瘤 T 细胞具有强大的细胞毒性。此外，与接受对照 T 细胞和 PBS 的小鼠相比，过继性 CD4CART 细胞转移显著抑制了 T 细胞淋巴瘤的肿瘤生长并提高了动物存活率。
CD4CART 细胞对 T 细胞淋巴瘤具有强大的细胞毒性作用。该研究为 CD4CART 介导的 T 细胞淋巴瘤治疗提供了实验基础。
