构建生产 L-苯丙氨酸衍生化合物的重组大肠杆菌。
Construction of recombinant Escherichia coli for production of L-phenylalanine-derived compounds.
机构信息
School of Biological Science and Technology, University of Jinan, Jinan, 250022, People's Republic of China.
出版信息
World J Microbiol Biotechnol. 2021 Apr 15;37(5):84. doi: 10.1007/s11274-021-03050-1.
L-phenylalanine is an important amino acid that is widely used in the fields of food flavors and pharmaceuticals. Apart from L-phenylalanine itself, various commercially valuable chemical compounds can also be generated via the L-phenylalanine biosynthesis pathway. Compared with direct extraction from plants or synthesis by chemical reaction, microbial production of L-phenylalanine -derived compounds can overcome the drawbacks of environmental pollution, low yield, and mixtures of stereoisomeric products. Accordingly, increasing intracellular levels of precursors, deregulating feedback inhibition and transcription repression, engineering global regulators and other effective strategies have been implemented to produce different L-phenylalanine -derived compounds in the excellent chassis host Escherichia coli. Finally, this review highlights principal strategies for improving the production of L-phenylalanine and/or its derivatives in E. coli, and discusses the future outlook for further enhancing the titer and yields of these compounds.
L-苯丙氨酸是一种重要的氨基酸，广泛应用于食品香料和制药领域。除了 L-苯丙氨酸本身外，通过 L-苯丙氨酸生物合成途径还可以生成各种具有商业价值的化合物。与直接从植物中提取或通过化学反应合成相比，微生物生产 L-苯丙氨酸衍生化合物可以克服环境污染、产量低和立体异构体产物混合物的缺点。因此，通过增加前体的细胞内水平、解除反馈抑制和转录抑制、工程全局调控因子等有效策略，在优秀底盘宿主大肠杆菌中生产不同的 L-苯丙氨酸衍生化合物。最后，本文重点介绍了提高大肠杆菌中 L-苯丙氨酸及其衍生物产量的主要策略，并讨论了进一步提高这些化合物的浓度和产率的未来展望。
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