Faculty of Science, Centre of the Region Haná for Biotechnological and Agricultural Research, Palacký University Olomouc, Olomouc 783 71, Czech Republic.
Institute of Experimental Botany (IEB), Czech Acad Sci, Centre of the Region Haná for Biotechnological and Agricultural Research (CRH), Olomouc 779 00, Czech Republic.
Plant Physiol. 2021 Jul 6;186(3):1526-1544. doi: 10.1093/plphys/kiab171.
The YODA (YDA) kinase pathway is intimately associated with the control of Arabidopsis (Arabidopsis thaliana) embryo development, but little is known regarding its regulators. Using genetic analysis, HEAT SHOCK PROTEIN 90 (HSP90) proteins emerge as potent regulators of YDA in the process of embryo development and patterning. This study is focused on the characterization and quantification of early embryonal traits of single and double hsp90 and yda mutants. HSP90s genetic interactions with YDA affected the downstream signaling pathway to control the development of both basal and apical cell lineage of embryo. Our results demonstrate that the spatiotemporal expression of WUSCHEL-RELATED HOMEOBOX 8 (WOX8) and WOX2 is changed when function of HSP90s or YDA is impaired, suggesting their essential role in the cell fate determination and possible link to auxin signaling during early embryo development. Hence, HSP90s together with YDA signaling cascade affect transcriptional networks shaping the early embryo development.
YODA (YDA) 激酶途径与拟南芥 (Arabidopsis thaliana) 胚胎发育的控制密切相关，但关于其调节剂知之甚少。本研究使用遗传分析，发现热休克蛋白 90 (HSP90) 蛋白在胚胎发育和模式形成过程中是 YDA 的有效调节剂。本研究重点在于对单突变体和双突变体 hsp90 和 yda 的早期胚胎特征进行定性和定量分析。HSP90 与 YDA 的遗传相互作用影响下游信号通路，从而控制胚胎的基细胞系和顶细胞系的发育。我们的结果表明，当 HSP90 或 YDA 的功能受到损害时，WOX8 和 WOX2 的时空表达发生改变，这表明它们在细胞命运决定中起重要作用，并且可能与早期胚胎发育过程中的生长素信号转导有关。因此，HSP90 与 YDA 信号级联一起影响塑造早期胚胎发育的转录网络。
