The Key Laboratory of Biotechnology for Medicinal Plant of Jiangsu Province, School of Life Science, Jiangsu Normal University , Xuzhou, Jiangsu 221116, People's Republic of China.
J Agric Food Chem. 2017 May 24;65(20):4121-4132. doi: 10.1021/acs.jafc.7b00228. Epub 2017 May 16.
In higher plants, seed size is central to many aspects in evolutionary fitness and is a crucial agricultural trait. In this study, Arabidopsis an3 (angustifolia3) mutants present with increased seed size. Target-gene analysis revealed that YDA, which encodes a mitogen-activated protein kinase kinase kinase, is a target gene of AN3. Indeed, the loss of YDA function decreases seed size. Furthermore, AN3 and YDA mutations both disrupt normal sucrose and glucose contents and cause altered seed size in an3 or yda mutants. With these results, we provide a molecular model in which soluble sugar accumulation might affect seed size regulation via the AN3-YDA gene cascade. Our findings guide the synthesis of a model that predicts the integration of soluble sugar accumulation at AN3 to control the establishment of seed size.
在高等植物中，种子大小是进化适应性的核心，也是一个关键的农业性状。在这项研究中，拟南芥 an3（angustifolia3）突变体的种子大小增加。靶基因分析表明，编码丝裂原活化蛋白激酶激酶激酶的 YDA 是 AN3 的靶基因。事实上，YDA 功能的丧失会降低种子大小。此外，AN3 和 YDA 突变都会破坏正常的蔗糖和葡萄糖含量，并导致 an3 或 yda 突变体的种子大小发生改变。有了这些结果，我们提供了一个分子模型，其中可溶性糖的积累可能通过 AN3-YDA 基因级联影响种子大小的调节。我们的研究结果指导了一个模型的合成，该模型预测了可溶性糖在 AN3 处的积累可以控制种子大小的建立。
