Bagherzadeh Afsaneh, Vaziri Hamidreza, Nasimi Fatemeh Sokouti, Ahmadian Shahin, Feyzi Adel, Farhadi Mehrdad, Yahyavi Fariba, Hashemi Behnam, Rahbarghazi Reza, Mahdipour Mahdi
Department of Biology, Faculty of Science, Guilan University, Rasht, Iran.
Stem Cell Research Center, Tabriz University of Medical Sciences, Tabriz, Iran.
Cardiovasc Toxicol. 2021 Jul;21(7):582-591. doi: 10.1007/s12012-021-09651-4. Epub 2021 Apr 15.
It has been shown that near all organs, especially the cardiovascular system, are affected by bacterial lipopolysaccharide via the activation of Toll-like receptor signaling pathways. Here, we tried to find the blunting effect of bacterial lipase on lipopolysaccharide (LPS)-induced cardiac tissue toxicity in chicken embryos. 7-day fertilized chicken eggs were divided randomly into different groups as follows; Control, Normal Saline, LPS (0.1, 0.5 and 1 mg/kbw), and LPS (0.1, 0.5 and 1 mg/kbw) plus 5 mg/ml Lipase. On day 17, the hearts were sampled. The expression of genes such as GATA4, NKX2.5, EGFR, TRIF, and NF-ƙB was monitored using real-time PCR analysis. Using western blotting, we measured NF-ƙB protein level. Total antioxidant capacity, glutathione peroxidase, and Catalase activity were also studied. Microvascular density and anterior wall thickness were monitored in histological samples using H&E staining. High dose of LPS (1 mg/kbw) increased the expression of TRIF but not NF-ƙB compared to the control group (p < 0.05). We found a statistically significant reduction in groups that received LPS + Lipase compared to the control and LPS groups (p < 0.05). Western blotting revealed that the injection of Lipase could reduce LPS-induced NF-ƙB compared to the control group (p < 0.05). The expression of GATA4, NKx2.5, and EGFR was not altered in the LPS group, while the simultaneous application of LPS and Lipase significantly reduced GATA4, NKx2.5, and EGFR levels below the control (p < 0.05). We found non-significant differences in glutathione peroxidase, and Catalase activity in all groups (p > 0.05), while total antioxidant capacity was increased in groups that received LPS + Lipase. Anterior wall thickness was diminished in LPS groups and the use of both lipase and LPS returned near-to-control values (p < 0.05). Despite a slight increase in microvascular density, we found statistically non-significant differences in all groups (p > 0.05). Bacterial lipase reduces detrimental effects of LPS on chicken embryo heart induced via Toll-like receptor signaling pathway.
研究表明，几乎所有器官，尤其是心血管系统，都会通过Toll样受体信号通路的激活而受到细菌脂多糖的影响。在此，我们试图探究细菌脂肪酶对脂多糖（LPS）诱导的鸡胚心脏组织毒性的钝化作用。将7日龄受精鸡蛋随机分为以下不同组：对照组、生理盐水组、LPS（0.1、0.5和1mg/kbw）组，以及LPS（0.1、0.5和1mg/kbw）加5mg/ml脂肪酶组。在第17天采集心脏样本。使用实时PCR分析监测GATA4、NKX2.5、EGFR、TRIF和NF-ƙB等基因的表达。通过蛋白质印迹法，我们测量了NF-ƙB蛋白水平。还研究了总抗氧化能力、谷胱甘肽过氧化物酶和过氧化氢酶活性。使用苏木精-伊红染色在组织学样本中监测微血管密度和前壁厚度。与对照组相比，高剂量LPS（1mg/kbw）增加了TRIF的表达，但未增加NF-ƙB的表达（p < 0.05）。我们发现，与对照组和LPS组相比，接受LPS + 脂肪酶的组有统计学意义的降低（p < 0.05）。蛋白质印迹法显示，与对照组相比，注射脂肪酶可降低LPS诱导的NF-ƙB（p < 0.05）。LPS组中GATA4、NKx2.5和EGFR的表达未改变，而同时应用LPS和脂肪酶显著降低了GATA4、NKx2.5和EGFR水平至对照组以下（p < 0.05）。我们发现所有组中谷胱甘肽过氧化物酶和过氧化氢酶活性无显著差异（p > 0.05），而接受LPS + 脂肪酶的组总抗氧化能力增加。LPS组前壁厚度减小，同时使用脂肪酶和LPS使其恢复到接近对照组的值（p < 0.05）。尽管微血管密度略有增加，但我们发现所有组在统计学上无显著差异（p > 0.05）。细菌脂肪酶可降低LPS通过Toll样受体信号通路对鸡胚心脏的有害影响。
