Oral and Maxillofacial Diseases, University of Helsinki, Helsinki, Finland.
Institute of Global Health, University of Heidelberg, Heidelberg, Germany.
PLoS One. 2020 Feb 21;15(2):e0228806. doi: 10.1371/journal.pone.0228806. eCollection 2020.
Periodontitis is associated with increased serum lipopolysaccharide (LPS) activity, which may be one mechanism linking periodontitis with the risk of cardiovascular diseases. As LPS-carrying proteins including lipoproteins modify LPS-activity, we investigated the determinants of serum LPS-neutralizing capacity (LPS-NC) in ischemic stroke. The association of LPS-NC and Aggregatibacter actinomycetemcomitans, a major microbial biomarker in periodontitis, was also investigated.
The assay to measure LPS-NC was set up by spiking serum samples with E. coli LPS. The LPS-NC, LPS-binding protein (LBP), soluble CD14 (sCD14), lipoprotein profiles, apo(lipoprotein) A-I, apoB, and phospholipid transfer protein (PLTP) activity, were determined in 98 ischemic stroke patients and 100 age- and sex-matched controls. Serum and saliva immune response to A. actinomycetemcomitans, its concentration in saliva, and serotype-distribution were examined.
LPS-NC values ranged between 51-83% in the whole population. Although several of the LPS-NC determinants differed significantly between cases and controls (PLTP, sCD14, apoA-I, HDL-cholesterol), the levels did not (p = 0.056). The main determinants of LPS-NC were i) triglycerides (β = -0.68, p<0.001), and ii) HDL cholesterol (0.260, <0.001), LDL cholesterol (-0.265, <0.001), PLTP (-0.196, 0.011), and IgG against A. actinomycetemcomitans (0.174, 0.011). Saliva A. actinomycetemcomitans concentration was higher [log mean (95% CI), 4.39 (2.35-8.19) vs. 10.7 (5.45-21) genomes/ml, p = 0.023) and serotype D more frequent (4 vs. 0%, p = 0.043) in cases than controls. Serotypeablity or serotypes did not, however, relate to the LPS-NC.
Serum LPS-NC comprised low PLTP-activity, triglyceride and LDL cholesterol concentrations, as well as high HDL cholesterol and IgG against A. actinomycetemcomitans. The present findings let us to conclude that LPS-NC did not associate with stroke.
牙周炎与血清脂多糖（LPS）活性增加有关，这可能是牙周炎与心血管疾病风险相关的机制之一。由于携带 LPS 的蛋白质（包括脂蛋白）会改变 LPS 的活性，我们研究了缺血性中风患者血清 LPS 中和能力（LPS-NC）的决定因素。还研究了 LPS-NC 与牙周炎主要微生物生物标志物聚集放线杆菌之间的关联。
通过向血清样本中加入大肠杆菌 LPS 来建立测量 LPS-NC 的测定方法。在 98 名缺血性中风患者和 100 名年龄和性别匹配的对照者中，测定了 LPS-NC、LPS 结合蛋白（LBP）、可溶性 CD14（sCD14）、脂蛋白谱、载脂蛋白（脂蛋白）A-I、载脂蛋白 B 和磷脂转移蛋白（PLTP）活性。检测了血清和唾液对聚集放线杆菌的免疫反应、唾液中的浓度和血清型分布。
整个人群的 LPS-NC 值范围在 51％-83％之间。尽管病例组和对照组之间的一些 LPS-NC 决定因素差异显著（PLTP、sCD14、apoA-I、HDL 胆固醇），但水平没有差异（p=0.056）。LPS-NC 的主要决定因素是 i）甘油三酯（β=-0.68，p<0.001），ii）HDL 胆固醇（0.260，<0.001），LDL 胆固醇（-0.265，<0.001），PLTP（-0.196，0.011）和针对聚集放线杆菌的 IgG（0.174，0.011）。与对照组相比，病例组唾液中聚集放线杆菌的浓度更高[对数平均值（95％CI），4.39（2.35-8.19）vs. 10.7（5.45-21）基因组/ml，p=0.023）和血清型 D 更为频繁（4 对 0％，p=0.043）。然而，血清型或血清型与 LPS-NC 无关。
血清 LPS-NC 包含低 PLTP 活性、甘油三酯和 LDL 胆固醇浓度，以及高 HDL 胆固醇和针对聚集放线杆菌的 IgG。本研究结果表明，LPS-NC 与中风无关。
