长链非编码 RNA XIST 通过负调控 miR-192 上调 TRIM25 在乙型肝炎病毒相关肝细胞癌中的表达。
LncRNA XIST upregulates TRIM25 via negatively regulating miR-192 in hepatitis B virus-related hepatocellular carcinoma.
机构信息
The People's Hospital of Lianshui County, Huai'an City, 223400, Jiangsu Province, People's Republic of China.
Department of Intervention, The Second People's Hospital of Huai'an City, Huai'an City, 223002, Jiangsu Province, People's Republic of China.
出版信息
Mol Med. 2021 Apr 15;27(1):41. doi: 10.1186/s10020-021-00278-3.
BACKGROUND
Long non-coding RNA (lncRNA) XIST has been implicated in the progression of a variety of tumor diseases. The purpose of this study was to explore the molecular role of lncRNA XIST in human hepatitis B virus (HBV)-related hepatocellular carcinoma (HCC).
METHODS
The expression levels of lncRNA XIST, miR-192 and TRIM25 in HBV-related HCC tissues and HepG2.2.15 cells were detected by qRT-PCR. Biological information and luciferin gene reporter assay were performed to detect the interaction among lncRNA XIST, miR-192 and TRIM25. CCk-8 assay, wound healing assay and colony formation assay were conducted to detect the proliferation and migration ability of HepG2.2.15 cells.
RESULTS
qRT-PCR results showed that the expression levels of lncRNA XIST were remarkably increased in HBV-related HCC tissues and HepG2.2.15 cells. In addition, miR-192 was a direct target gene of lncRNA XIST, and the expression of miR-192 and lncRNA XIST were negatively correlated. Moreover, overexpression of miR-192 observably inhibited the proliferation and migration of HCC cells, while overexpression of lncRNA XIST showed an opposite effect. Furthermore, TRIM25 was a direct target of miR-192, and lncRNA XIST could up-regulate the expression of TRIM25 by targeting miR-192.
CONCLUSION
LncRNA XIST could up-regulate the expression of TRIM25 by targeting and binding to miR-192, thus accelerating the occurrence and development of HCC.
背景
长链非编码 RNA（lncRNA）XIST 参与多种肿瘤疾病的进展。本研究旨在探讨 lncRNA XIST 在乙型肝炎病毒（HBV）相关肝细胞癌（HCC）中的分子作用。
方法
通过 qRT-PCR 检测 HBV 相关 HCC 组织和 HepG2.2.15 细胞中 lncRNA XIST、miR-192 和 TRIM25 的表达水平。进行生物信息学和荧光素酶基因报告实验，以检测 lncRNA XIST、miR-192 和 TRIM25 之间的相互作用。通过 CCK-8 测定、划痕愈合测定和集落形成测定来检测 HepG2.2.15 细胞的增殖和迁移能力。
结果
qRT-PCR 结果显示，lncRNA XIST 在 HBV 相关 HCC 组织和 HepG2.2.15 细胞中的表达水平显著升高。此外，miR-192 是 lncRNA XIST 的直接靶基因，miR-192 和 lncRNA XIST 的表达呈负相关。此外，miR-192 的过表达显著抑制 HCC 细胞的增殖和迁移，而过表达 lncRNA XIST 则表现出相反的效果。此外，TRIM25 是 miR-192 的直接靶基因，lncRNA XIST 可以通过靶向结合 miR-192 而上调 TRIM25 的表达。
结论
lncRNA XIST 通过靶向结合 miR-192 而上调 TRIM25 的表达，从而加速 HCC 的发生和发展。
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