Department of Cariology, Operative Dentistry and Dental Public Health, Indiana University School of Dentistry (IUSD), 1121 W Michigan St, DS406, Indianapolis, IN, 46202, USA.
Department of Endodontics, Indiana University School of Dentistry (IUSD), Indianapolis, IN, 46202, USA.
Sci Rep. 2021 Apr 15;11(1):8306. doi: 10.1038/s41598-021-87922-4.
Root canal disinfection is of utmost importance in the success of the treatment, thus, a novel method for achieving root canal disinfection by electromagnetic waves, creating a synergistic reaction via electric and thermal energy, was created. To study electromagnetic stimulation (EMS) for the disinfection of root canal in vitro, single rooted teeth were instrumented with a 45.05 Wave One Gold reciprocating file. Specimens were sterilized and inoculated with Enterococcus faecalis ATCC 29,212, which grew for 15 days to form an established biofilm. Samples were treated with 6% sodium hypochlorite (NaOCl), 1.5% NaOCl 1.5% NaOCl with EMS, 0.9% saline with EMS or 0.9% saline. After treatments, the colony forming units (CFU) was determined. Data was analyzed by Wilcoxon Rank Sums Test (α = 0.05). One sample per group was scored and split for confocal laser scanning microscopy imaging. There was a significant effect with the use of NaOCl with or without EMS versus 0.9% saline with or without EMS (p = 0.012 and 0.003, respectively). CFUs were lower when using 0.9% saline with EMS versus 0.9% saline alone (p = 0.002). Confocal imaging confirmed CFU findings. EMS with saline has an antibiofilm effect against E. faecalis and can potentially be applied for endodontic disinfection.
根管消毒在治疗成功中至关重要，因此，开发了一种通过电磁波实现根管消毒的新方法，通过电能和热能产生协同反应。为了研究电磁刺激（EMS）对体外根管的消毒作用，使用 45.05 Wave One Gold 往复锉对单根牙进行根管预备。将标本进行消毒并接种粪肠球菌 ATCC 29212，培养 15 天形成成熟生物膜。将样本用 6%次氯酸钠（NaOCl）、1.5% NaOCl 与 EMS、0.9%盐水与 EMS 或 0.9%盐水进行处理。处理后，测定集落形成单位（CFU）。采用 Wilcoxon 秩和检验（α=0.05）对数据进行分析。每组取一个样本进行共聚焦激光扫描显微镜成像评分和分割。与 0.9%盐水与 EMS 或 0.9%盐水相比，使用 NaOCl 与 EMS 或不使用 EMS 均有显著效果（分别为 p=0.012 和 0.003）。与单独使用 0.9%盐水相比，使用 0.9%盐水与 EMS 时 CFU 更低（p=0.002）。共聚焦成像证实了 CFU 的发现。盐水与 EMS 具有抗粪肠球菌生物膜的作用，可能适用于牙髓消毒。
