Tennert Christian, Feldmann Katharina, Haamann Edwina, Al-Ahmad Ali, Follo Marie, Wrbas Karl-Thomas, Hellwig Elmar, Altenburger Markus J
Department of Operative Dentistry and Periodontology, University of Freiburg - Medical Center, Hugstetter Str, 55, 79106 Freiburg, Germany.
BMC Oral Health. 2014 Nov 4;14:132. doi: 10.1186/1472-6831-14-132.
To determine the antibacterial effect of photodynamic Therapy on Enterococcus faecalis (E. faecalis) biofilms in experimentally infected human root canals in primary infections and endodontic retreatments.
One hundred and sixty single-rooted extracted teeth with one root canal were prepared using ProTaper instruments. Seventy specimens were left without root canal filling and autoclaved. The root canals of another 70 specimens were filled with Thermafil and AH Plus and the root canal fillings were removed after 24 hours using ProTaper D files and plasma sterilized. The specimens were infected with a clinical isolate of E. faecalis for 72 hours. Samples were taken using sterile paper points to determine the presence of E. faecalis in the root canals. The specimens were randomly divided into groups according to their treatment with 20 teeth each and a control. In the PDT group the teeth were treated using PDT, consisting of the photosensitizer toluidine blue and the PDT light source at 635 nm. In the NaOCl (sodium hypochlorite) group the root canals were rinsed with 10 mL of 3% NaOCl. In the NaOCl-PDT group the root canals were rinsed with 10 mL of 3% of sodium hypochlorite and then treated with PDT. Samples were taken after treatments using sterile paper points. Additionally, remaining root canal filling material was recovered from the root canal walls. Survival fractions of the samples were calculated by counting colony-forming units. A one-way analysis of variance (ANOVA) was applied to the data to assess the effect of different treatment techniques.
Antimicrobial treatment of root canals caused a significant reduction of bacterial load in all groups. NaOCl irrigation eliminated E. faecalis most effectively. PDT alone was less effective compared to NaOCl irrigation and the combination of NaOCl irrigation and PDT. CFU levels recovered from the filling material after NaOCl irrigation of the root canals were 10fold higher compared to PDT and the combination of NaOCl irrigation and PDT.
Photodynamic therapy killed E. faecalis in experimental primary endodontic infections and retreated human root canals. PDT is an effective supplement in root canal disinfection, especially in endodontic retreatments.
确定光动力疗法对原发性感染和根管再治疗中实验性感染人根管内粪肠球菌生物膜的抗菌效果。
使用ProTaper器械对160颗单根管离体牙进行预备。70个标本不进行根管充填并高压灭菌。另外70个标本的根管用Thermafil和AH Plus充填，24小时后用ProTaper D锉去除根管充填物并进行等离子体灭菌。将标本用粪肠球菌临床分离株感染72小时。使用无菌纸尖取样以确定根管内粪肠球菌的存在。根据治疗方法将标本随机分为每组20颗牙的组和一个对照组。在光动力疗法（PDT）组中，使用PDT对牙齿进行治疗，包括光敏剂甲苯胺蓝和635nm的PDT光源。在次氯酸钠（NaOCl）组中，用10mL 3%的NaOCl冲洗根管。在NaOCl-PDT组中，用10mL 3%的次氯酸钠冲洗根管，然后进行PDT治疗。治疗后使用无菌纸尖取样。此外，从根管壁回收剩余的根管充填材料。通过计数菌落形成单位计算样本的存活分数。对数据应用单因素方差分析（ANOVA）以评估不同治疗技术的效果。
根管的抗菌治疗使所有组的细菌载量显著降低。NaOCl冲洗最有效地消除了粪肠球菌。与NaOCl冲洗以及NaOCl冲洗和PDT联合使用相比，单独使用PDT效果较差。根管经NaOCl冲洗后从充填材料中回收菌落形成单位（CFU）水平比PDT以及NaOCl冲洗和PDT联合使用高10倍。
光动力疗法杀死了实验性原发性牙髓感染和再治疗的人根管内的粪肠球菌。PDT是根管消毒的有效补充，尤其是在根管再治疗中。
