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Ag 依赖性 B 细胞分化过程中动态的细胞内代谢细胞信号特征。

Dynamic Intracellular Metabolic Cell Signaling Profiles During Ag-Dependent B-Cell Differentiation.

机构信息

Department of Medicine and Cytometry General Service-Nucleus, CIBERONC, Cancer Research Centre (IBMCC/CSIC/USAL/IBSAL), Salamanca, Spain.

Proteomics Unit, Cancer Research Centre (IBMCC/CSIC/USAL/IBSAL), Salamanca, Spain.

出版信息

Front Immunol. 2021 Mar 30;12:637832. doi: 10.3389/fimmu.2021.637832. eCollection 2021.

DOI:10.3389/fimmu.2021.637832
PMID:33859640
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC8043114/
Abstract

Human B-cell differentiation has been extensively investigated on genomic and transcriptomic grounds; however, no studies have accomplished so far detailed analysis of antigen-dependent maturation-associated human B-cell populations from a proteomic perspective. Here, we investigate for the first time the quantitative proteomic profiles of B-cells undergoing antigen-dependent maturation using a label-free LC-MS/MS approach applied on 5 purified B-cell subpopulations (naive, centroblasts, centrocytes, memory and plasma B-cells) from human tonsils (data are available ProteomeXchange with identifier PXD006191). Our results revealed that the actual differences among these B-cell subpopulations are a combination of expression of a few maturation stage-specific proteins within each B-cell subset and maturation-associated changes in relative protein expression levels, which are related with metabolic regulation. The considerable overlap of the proteome of the 5 studied B-cell subsets strengthens the key role of the regulation of the stoichiometry of molecules associated with metabolic regulation and programming, among other signaling cascades (such as antigen recognition and presentation and cell survival) crucial for the transition between each B-cell maturation stage.

摘要

人类 B 细胞分化已在基因组和转录组水平上得到广泛研究；然而，迄今为止，尚无研究从蛋白质组学的角度完成对抗原依赖性成熟相关的人类 B 细胞群体的详细分析。在这里，我们首次使用无标记 LC-MS/MS 方法研究了抗原依赖性成熟过程中 B 细胞的定量蛋白质组学图谱，该方法应用于 5 种从人扁桃体纯化的 B 细胞亚群（幼稚细胞、中心母细胞、中心细胞、记忆 B 细胞和浆细胞）（数据可在 ProteomeXchange 中以标识符 PXD006191 获得）。我们的结果表明，这些 B 细胞亚群之间的实际差异是每个 B 细胞亚群中少数成熟阶段特异性蛋白的表达与与代谢调节相关的相对蛋白表达水平的成熟相关变化的组合。5 种研究的 B 细胞亚群的蛋白质组具有相当大的重叠性，这加强了与代谢调节和编程相关的分子的化学计量调节以及其他信号级联（如抗原识别和呈递以及细胞存活）在每个 B 细胞成熟阶段之间的过渡中的关键作用。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f574/8043114/5ad4ca5baf14/fimmu-12-637832-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f574/8043114/ecbbe62caf4c/fimmu-12-637832-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f574/8043114/15c1af1abc93/fimmu-12-637832-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f574/8043114/bffda3584dd4/fimmu-12-637832-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f574/8043114/5ad4ca5baf14/fimmu-12-637832-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f574/8043114/ecbbe62caf4c/fimmu-12-637832-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f574/8043114/15c1af1abc93/fimmu-12-637832-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f574/8043114/bffda3584dd4/fimmu-12-637832-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f574/8043114/5ad4ca5baf14/fimmu-12-637832-g004.jpg

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