Le Tho Son, Nguyen T T Hang, Thi Mai Huong Bui, Nguyen H Gam, Ha B Hong, Nguyen Van Sang, Nguyen Minh Hung, Nguyen Huy-Hoang, Wang John
College of Forestry Biotechnology, Vietnam National University of Forestry, Hanoi, Vietnam.
Faculty of Biology, VNU University of Science, Vietnam National University, Hanoi, Vietnam.
J Nematol. 2021 Apr 1;53. doi: 10.21307/jofnem-2021-036. eCollection 2021.
The study of species biodiversity within the genus of nematodes would be facilitated by the isolation of as many species as possible. So far, over 50 species have been found, usually associated with decaying vegetation or soil samples, with many from Africa, South America and Southeast Asia. Scientists based in these regions can contribute to sampling and their proximity would allow intensive sampling, which would be useful for understanding the natural history of these species. However, severely limited research budgets are often a constraint for these local scientists. In this study, we aimed to find a more economical, alternative growth media to rear and related species. We tested 25 media permutations using cheaper substitutes for the reagents found in the standard nematode growth media (NGM) and found three media combinations that performed comparably to NGM with respect to the reproduction and longevity of . These new media should facilitate the isolation and characterization of and other free-living nematodes for the researchers in the poorer regions such as Africa, South America, and Southeast Asia where nematode diversity appears high.
尽可能多地分离出线虫物种将有助于对线虫属内的物种生物多样性进行研究。到目前为止，已经发现了50多种线虫，它们通常与腐烂的植被或土壤样本有关，其中许多来自非洲、南美洲和东南亚。这些地区的科学家可以参与采样，而且由于距离较近，可以进行密集采样，这对于了解这些物种的自然历史很有用。然而，研究预算严重受限往往是这些当地科学家面临的一个制约因素。在本研究中，我们旨在找到一种更经济的替代生长培养基来培养[线虫名称]及相关物种。我们使用标准线虫生长培养基（NGM）中试剂的更便宜替代品测试了25种培养基组合，发现了三种培养基组合，它们在[线虫名称]的繁殖和寿命方面与NGM表现相当。这些新培养基应该有助于为非洲、南美洲和东南亚等较贫困地区的研究人员分离和鉴定[线虫名称]及其他自由生活线虫，这些地区的线虫多样性似乎很高。
