30S核糖体蛋白S1参与聚（U）指导的聚苯丙氨酸合成。

Involvement of 30S ribosomal protein S1 in poly(U)-directed polyphenylalanine synthesis.

作者信息

Yokota T, Arai K I, Kaziro Y

出版信息

J Biochem. 1977 Nov;82(5):1485-9. doi: 10.1093/oxfordjournals.jbchem.a131838.

DOI:10.1093/oxfordjournals.jbchem.a131838

Abstract

The effect of 30S ribosomal protein S1 on poly(U)-directed polyphenylalanine synthesis was studied using a highly purified cell-free system which was devoid of endogenous S1. The system consisted of homogeneous preparations of EF-Tu, EF-Ts, and EF-G, and 70S ribosomes from which protein S1 had been removed by poly(U)-cellulose column chromatography. It was found that protein S1 was indispensable for translation of poly(U) by an S1-depleted system at low concentrations of poly(U). On the other hand, at higher concentrations of poly(U), a considerable amount of polyphenylalanine was synthesized in the absence of added S1. The stimulatory effect of S1 was observed at all Mg2+ concentrations examined but was most pronounced at 10 mM Mg2+. Some physicochemical properties of the protein were also studied. It was demonstrated that the protein has an elongated shape with an axial ratio of approximately 8.5.

摘要

利用高度纯化的无细胞系统研究了30S核糖体蛋白S1对聚（U）指导的聚苯丙氨酸合成的影响，该系统不含内源性S1。该系统由EF-Tu、EF-Ts和EF-G的均一制剂以及通过聚（U）-纤维素柱色谱去除了蛋白S1的70S核糖体组成。结果发现，在低浓度聚（U）下，蛋白S1对于S1缺失系统翻译聚（U）是必不可少的。另一方面，在较高浓度的聚（U）下，在不添加S1的情况下也能合成相当数量的聚苯丙氨酸。在所有检测的Mg2+浓度下均观察到S1的刺激作用，但在10 mM Mg2+时最为明显。还研究了该蛋白的一些物理化学性质。结果表明，该蛋白呈细长形状，轴比约为8.5。