Institute of Photomedicine, Shanghai Skin Disease Hospital, Tongji University School of Medicine, Shanghai, China.
Exp Dermatol. 2021 Jul;30(7):923-931. doi: 10.1111/exd.14357. Epub 2021 May 9.
Acne vulgaris is a chronic inflammatory cutaneous disease. 5-Aminolaevulinic acid photodynamic therapy (ALA-PDT) is a novel and effective approach for severe acne vulgaris treatment. However, its specific treatment mechanism still remains unclear. In the present study, we investigated the potential mechanism of how ALA-PDT regulated intense inflammatory response in acne vulgaris. It appeared that ALA-PDT suppresses proliferation and lipid secretion of primary human sebocytes. Besides, ALA-PDT could up-regulate the expression of CXCL8 in vivo and in vitro, amplifying the inflammatory response by recruiting T cells, B cells, neutrophils and macrophages. We also found that ALA-PDT elevated the expression of CXCL8 via p38 pathway. SB203580, a p38 pathway inhibitor, decreased the expression of CXCL8 in sebocytes after ALA-PDT. These findings indicate that ALA-PDT amplifies the intense inflammatory response in the treatment of acne vulgaris via CXCL8. Our data decipher the mechanism of intense inflammatory response after ALA-PDT for acne vulgaris.
寻常痤疮是一种慢性炎症性皮肤病。5-氨基酮戊酸光动力疗法（ALA-PDT）是一种治疗严重寻常痤疮的新方法，疗效确切。然而，其具体的治疗机制尚不清楚。在本研究中，我们研究了 ALA-PDT 调节寻常痤疮中强烈炎症反应的潜在机制。结果表明，ALA-PDT 可抑制原代人皮脂腺细胞的增殖和脂质分泌。此外，ALA-PDT 可以在体内和体外上调 CXCL8 的表达，通过招募 T 细胞、B 细胞、中性粒细胞和巨噬细胞来放大炎症反应。我们还发现，ALA-PDT 通过 p38 通路上调 CXCL8 的表达。p38 通路抑制剂 SB203580 可降低 ALA-PDT 后皮脂腺细胞中 CXCL8 的表达。这些发现表明，ALA-PDT 通过 CXCL8 放大寻常痤疮治疗中的强烈炎症反应。我们的数据揭示了 ALA-PDT 治疗寻常痤疮后强烈炎症反应的机制。
