Shimizu K, Tahara K, Nakajyo S, Urakawa N
Department of Veterinary Pharmacology, Nippon Veterinary and Zootechnical College, Tokyo, Japan.
Nihon Heikatsukin Gakkai Zasshi. 1988 Jan;24(1):13-21. doi: 10.1540/jsmr1965.24.13.
In the present experiments, we investigated contractions of guinea-pig vas deferens or seminal vesicle in various kinds of high K+ solution. The vas deferens showed a phasic contraction followed by a tonic one in hyperosmotic 65 mM KCl (hyper-65K+), isosmotic 60 mM or 120 mM KCl solution. However, isosmotic 154 mM KCl (iso-154K+) solution induced a large phasic contraction followed by a gradual relaxation. Applications of the high K+ solutions to the preparation of seminal vesicle showed almost similar results in tension to those of the vas deferens. In the vas deferens or seminal vesicle, an application of hyper-65K+ solution significantly decreased the relative value for the wet weight of the tissue at 120 min, but that of iso-154K+ solution did not affect the wet weight of both the muscles. In the vas deferens, an hyperosmotic addition of sucrose at a concentration of 25 to 100 mM slightly prevented the tension decreased by the iso-154K+ solution and slightly decreased the relative wet weight. In the seminal vesicle, increasing sucrose concentration significantly increased the tension, but decreased the relative wet weight. In both the muscles of vas deferens and seminal vesicle, an addition of pyrvate or oxalacetate (5.5 mM) to the iso-154K+ solution without glucose maintained the muscle tension at a maximal level in the iso-154K+ solution with glucose for 120 min. Further, the tension decrease in both the muscles induced by iso-154K+ solution was also prevented by hyperosmotic application of NaCl. From these results, it is suggested that the tension decrease by high K+, Na+-deficient solution in the vas deferens is mainly caused by an inhibition of glucose utilization by Na+-deficiency in the medium, however, the seminal vesicle is probably due to the cell swelling and partly the inhibition of glucose utilization by it.
在本实验中，我们研究了豚鼠输精管或精囊在各种高钾溶液中的收缩情况。在高渗65 mM KCl（高-65K +）、等渗60 mM或120 mM KCl溶液中，输精管呈现出先相性收缩后强直性收缩的情况。然而，等渗154 mM KCl（等-154K +）溶液诱导出大的相性收缩，随后逐渐松弛。将高钾溶液应用于精囊制备时，其张力变化结果与输精管几乎相似。在输精管或精囊中，应用高-65K +溶液在120分钟时显著降低了组织湿重的相对值，但等-154K +溶液对两种肌肉的湿重均无影响。在输精管中，添加浓度为25至100 mM的蔗糖可略微防止等-154K +溶液引起的张力降低，并略微降低相对湿重。在精囊中，增加蔗糖浓度可显著增加张力，但降低相对湿重。在输精管和精囊的肌肉中，向不含葡萄糖的等-154K +溶液中添加丙酮酸或草酰乙酸（5.5 mM）可使肌肉张力在含葡萄糖的等-154K +溶液中维持120分钟的最大水平。此外，等渗应用NaCl也可防止等-154K +溶液引起的两种肌肉的张力降低。从这些结果表明，输精管中高钾、低钠溶液引起的张力降低主要是由于培养基中钠缺乏抑制了葡萄糖利用，然而，精囊可能是由于细胞肿胀以及部分由其对葡萄糖利用的抑制所致。
