Carroll P M
J Gen Physiol. 1969 May;53(5):590-607. doi: 10.1085/jgp.53.5.590.
The role of propagated activity in the responses to agonist drugs was studied for the rat uterus and vas deferens. Hypertonic solutions were used to inhibit propagation of activity by shrinking cells. Tissue weight was used to indicate cell volume. Hypertonic solutions after 10 min caused weight loss and reduced the size of contractions in response to submaximal doses of drugs, to KCl, and to external electrical stimulation. Contractions in response to KCl and drugs were diminished to a similar degree in the vas deferens, but in the uterus, drug contractions were depressed much more. Prolonged action of hypertonic solution also differed for the two tissues. In the uterus, weight changes correlated with changes in size of the drug-induced contractions. Uterine contractions reduced in hypertonic solution could be increased by using supramaximal doses of drug. When stimulation was applied to one end of the uterus in a three compartment bath, propagation of spontaneous drug- and KCl-induced contraction occurred, but it was prevented by placing hypertonic solution in the center compartment. An increase of the KCl to 44 mM in the hypertonic solution restored propagation. These experiments yielded no evidence of propagated responses in the rat vas deferens. It was concluded that propagated activity plays a role in drug-induced contractions in the rat uterus but not in the rat vas deferens. Hyperpolarization of shrunken cells might be involved in inhibition of propagation by hypertonic solutions.
研究了扩布性活动在大鼠子宫和输精管对激动剂药物反应中的作用。使用高渗溶液通过使细胞皱缩来抑制活动的扩布。组织重量用于指示细胞体积。10分钟后的高渗溶液导致重量减轻，并减少了对次最大剂量药物、氯化钾和外部电刺激的收缩幅度。输精管中对氯化钾和药物的收缩减少程度相似，但在子宫中，药物引起的收缩受到的抑制更大。高渗溶液的延长作用在两种组织中也有所不同。在子宫中，重量变化与药物诱导收缩的幅度变化相关。高渗溶液中减少的子宫收缩可以通过使用超最大剂量的药物来增加。当在三室浴中对子宫的一端施加刺激时，自发的药物和氯化钾诱导的收缩会发生扩布，但通过在中间隔室放置高渗溶液可以阻止这种扩布。将高渗溶液中的氯化钾浓度增加到44 mM可恢复扩布。这些实验没有提供大鼠输精管中存在扩布性反应的证据。得出的结论是，扩布性活动在大鼠子宫的药物诱导收缩中起作用，但在大鼠输精管中不起作用。皱缩细胞的超极化可能参与了高渗溶液对扩布的抑制作用。
